Mates A, Schaffer M
Public Health Laboratory, Ministry of Health, Haifa, Israel.
Microbios. 1988;53(216-217):161-5.
A simple rapid method was developed for counting Escherichia coli in sea water using a membrane filter procedure. Following filtration the membrane filter was incubated on mFC agar medium for 24 +/- 2 h at 44.5 +/- 0.2 degrees C for determination of faecal coliforms. An in situ test for the determination of E. coli was carried out by transferring the membrane filter to nutrient agar containing 4,methyl-umbiliferyl-B-D, glucoronide, followed by incubation for 3 h at 35 degrees C. The E. coli colonies were detected by fluorescence under long wavelength UV light. Extensive biochemical confirmation tests on the isolates showed that all the fluorescence colonies which were either lactose positive or negative were E. coli.
开发了一种使用膜过滤程序对海水中大肠杆菌进行计数的简单快速方法。过滤后，将膜过滤器在mFC琼脂培养基上于44.5±0.2℃下孵育24±2小时，以测定粪大肠菌群。通过将膜过滤器转移到含有4-甲基伞形酮-β-D-葡萄糖醛酸苷的营养琼脂上，然后在35℃下孵育3小时，进行大肠杆菌的原位检测。在长波长紫外光下通过荧光检测大肠杆菌菌落。对分离株进行的广泛生化确认试验表明，所有乳糖阳性或阴性的荧光菌落均为大肠杆菌。
