Rice E W, Allen M J, Edberg S C
Risk Reduction Engineering Laboratory, U.S. Environmental Protection Agency, Cincinnati, Ohio 45268.
Appl Environ Microbiol. 1990 May;56(5):1203-5. doi: 10.1128/aem.56.5.1203-1205.1990.
In 1976, Kilian and Bulow described the association of beta-glucuronidase with the genus Escherichia (97% positive) and suggested that a beta-glucuronidase assay would be a useful identification test. Since that report, papers about the sensitivity and specificity of this enzyme for the identification of Escherichia coli from clinical sources, food, seawater, potable-water supplies, and various environmental sources have appeared. A study was undertaken to determine the efficacy and specificity of the defined-substrate technology beta-glucuronidase (Colilert) assay for the identification of this species from fecal samples. A total of 460 human, 105 cow, and 55 horse E. coli isolates were tested. Results showed 95.5% beta-glucuronidase-positive isolates in 24 h and 99.5% positive after 28 h of incubation. Only one E. coli isolate was negative. There were no significant differences in the percentage of beta-glucuronidase-positive isolates among the human or animal isolates. There were no non-E. coli isolates that were positive. All subjects carried beta-glucuronidase-positive E. coli.
1976年，基利安和布洛描述了β-葡萄糖醛酸酶与埃希氏菌属的关联（阳性率97%），并提出β-葡萄糖醛酸酶检测将是一种有用的鉴定试验。自该报告发表以来，出现了许多关于该酶从临床样本、食品、海水、饮用水供应及各种环境来源中鉴定大肠杆菌的敏感性和特异性的论文。开展了一项研究，以确定用于从粪便样本中鉴定该菌的特异性底物技术β-葡萄糖醛酸酶（Colilert）检测的有效性和特异性。共检测了460株人源、105株牛源和55株马源的大肠杆菌分离株。结果显示，培养24小时后，95.5%的分离株β-葡萄糖醛酸酶呈阳性，培养28小时后，99.5%呈阳性。只有一株大肠杆菌分离株为阴性。人源或动物源分离株中β-葡萄糖醛酸酶阳性分离株的百分比无显著差异。没有非大肠杆菌分离株呈阳性。所有受试者均携带β-葡萄糖醛酸酶阳性的大肠杆菌。
