Zhang Huimin, Li Quan, Qiao Guang, Qiu Zhilang, Wen Zhuang, Wen Xiaopeng
Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute of Agro-bioengineering, College of Life Science, Guizhou University, Guiyang 550025, P. R. China.
Institute for Forest Resources & Environment of Guizhou, College of Forestry, Guizhou University, Guiyang 550025, P. R. China.
Anal Methods. 2020 Jun 18;12(23):3004-3013. doi: 10.1039/d0ay00718h.
To study the application value of sweet cherry leaves before abscission, a supercritical carbon dioxide (SFE-CO2) extraction method was established for sweet cherry (Prunus avium L.) leaves. The extraction temperature, pressure and time were optimized with a Box-Behnken design, and the optimal conditions were 43 °C, 30 MPa, and 120 min, resulting in a yield of 2.52 ± 0.261% (w/w), which agrees well with the predicted value. The yield of the sweet cherry leaf extracts obtained by methanol solvent extraction is 2.03% (w/w); the SFE-CO2 extraction method was more efficient and had a higher yield than the methanol solvent extraction method, and the adverse effects of organic solvent residues and high temperatures on the product during traditional solvent extraction were avoided, resulting in a higher quality product. UPLC-MS/MS in negative ion mode provided 56 identifiable chromatographic peaks, including those of 31 acids (13.25%), 5 sugars (15.94%), 2 alcohols (6.19%), and 18 other compounds (3.86%). The fragmentation pathways of the 7 main components in the sweet cherry leaf extract were identified. The carbohydrates and bioactive substances in the extracts obtained from sweet cherry leaves suggested the potential use of sweet cherry leaves in the food and medical industries.
为研究甜樱桃叶片脱落前的应用价值，建立了甜樱桃（Prunus avium L.）叶片的超临界二氧化碳（SFE - CO2）萃取方法。采用Box - Behnken设计对萃取温度、压力和时间进行优化，最佳条件为43℃、30MPa和120min，产率为2.52±0.261%（w/w），与预测值吻合良好。甲醇溶剂萃取法得到的甜樱桃叶提取物产率为2.03%（w/w）；SFE - CO2萃取法比甲醇溶剂萃取法更高效且产率更高，避免了传统溶剂萃取过程中有机溶剂残留和高温对产品的不利影响，从而得到更高质量的产品。负离子模式下的超高效液相色谱 - 串联质谱（UPLC - MS/MS）提供了56个可识别的色谱峰，包括31种酸（13.25%）、5种糖（15.94%）、2种醇（6.19%）和18种其他化合物（3.86%）。确定了甜樱桃叶提取物中7种主要成分的裂解途径。甜樱桃叶提取物中的碳水化合物和生物活性物质表明甜樱桃叶在食品和医药行业具有潜在用途。
