Beijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Microb Drug Resist. 2021 Apr;27(4):476-484. doi: 10.1089/mdr.2020.0212. Epub 2020 Sep 15.
and co-harboring isolates have been reported, usually reside on different plasmids, suggesting co-transfer possibility of the two genes from separate donors to the same recipient strain. This study aims at screening and characterization of carrying Enterobacteriaceae in Northern China, and studying the transfer ability of alone and in company with from a second donor. Three strains and one strain carrying gene were screened out from 1992 isolates in our study. Co-existence of multiple resistance genes was found in the -carrying strains, but none of them carried . One demonstrated an single nucleotide polymorphism (SNP) (A-G) at -10 region of , and one showed 2 SNPs (G-T and G-A) in the Shine-Dalgarno sequence-like region of . The gene was located on plasmids of about 33-276 kb, and capable of transferring alone in three out of four -positive isolates by conjugation. Co-transfer ability analysis demonstrated that from 13-68, which could not be transferred alone to C600, was successfully transferred in company with from ATCC BAA-2146. showed low incidence in our Enterobacteriaceae isolates. Co-transfer ability of and NDM-1 from separate donors provides direct evidence for the emergence of the and bla co-harboring isolates.
并且已经报道了共分离株，它们通常位于不同的质粒上，这表明这两个基因可能来自不同供体转移到同一受体菌株。本研究旨在筛选和鉴定中国北方携带 基因的肠杆菌科细菌，并研究 基因和来自第二个供体的 基因单独和共同转移的能力。从本研究的 1992 株分离株中筛选出 3 株携带 基因的 株和 1 株携带 基因的 株。在携带 基因的菌株中发现了多种耐药基因的共存，但它们都不携带 基因。一株 基因在 -10 区域发生单核苷酸多态性（SNP）（A-G），一株 在 Shine-Dalgarno 序列样区有 2 个 SNP（G-T 和 G-A）。 基因位于约 33-276kb 的质粒上，在 4 株 阳性分离株中，有 3 株通过接合可以单独转移。共转移能力分析表明，来自 13-68 的 基因不能单独转移到 C600，但可以与来自 ATCC BAA-2146 的 基因共同转移。 在本研究的肠杆菌科分离株中发生率较低。来自不同供体的 基因和 NDM-1 的共转移能力为 基因和 bla 共分离株的出现提供了直接证据。
