Xu Shuguang, Tu Jinjing, Zhang Liang, Chen Yinv, Dong Xiaoqi, Chi Xiaohui, Xu Hao
Department of Pulmonary and Critical Care Medicine, Ningbo Medical Center Lihuili Hospital, Ningbo, People's Republic of China.
Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, People's Republic of China.
Infect Drug Resist. 2022 Jun 2;15:2835-2841. doi: 10.2147/IDR.S360353. eCollection 2022.
Nosocomial infections caused by New Delhi metallo-β-lactamase (NDM)-producing bacteria are prevalent worldwide. However, such diseases caused by NDM-producing had never been reported. Our study aimed to elucidate the genomic characteristics of NDM-1-producing isolated from hospital patients.
Bacterial genomic features and possible origins were assessed by whole-genome sequencing (WGS) and phylogenetic analysis. Subsequent investigations include antimicrobial susceptibility testing and multilocus sequence typing (MLST).
We identified here two NDM-1-producing isolates from bacteremia. Susceptibility testing showed that two isolates were multi-drug resistant and shared a similar resistance profile and were only sensitive to amikacin and trimethoprim/sulfamethoxazole. Both isolates carry the carbapenem resistance gene and also have antibiotic resistance genes such as β-lactams, AmpC enzymes, macrolides, aminoglycosides, and quinolones. S1-PFGE and Southern blot analysis were negative. Whole-genome sequencing and comparative analysis revealed that these two isolates shared a close relationship.
To the best of our knowledge, this work describes the first detection of non-plasmid encoded in . The isolated in this study has a broad drug resistance spectrum. Phenotypic and molecular analysis indicated the two isolates belong to the same clone. Routine genomic surveillance of this species is now necessary to effectively curb the further dissemination of carbapenem-resistant bacteria in the region.
由产新德里金属β-内酰胺酶（NDM）细菌引起的医院感染在全球范围内普遍存在。然而，此前从未有过由产NDM细菌引起此类疾病的报道。我们的研究旨在阐明从医院患者中分离出的产NDM-1细菌的基因组特征。
通过全基因组测序（WGS）和系统发育分析评估细菌的基因组特征和可能的来源。后续研究包括抗菌药物敏感性测试和多位点序列分型（MLST）。
我们在此从菌血症中鉴定出两株产NDM-1细菌分离株。药敏试验表明，这两株分离株对多种药物耐药，具有相似的耐药谱，仅对阿米卡星和复方磺胺甲恶唑敏感。两株细菌均携带碳青霉烯耐药基因，还具有β-内酰胺类、AmpC酶、大环内酯类、氨基糖苷类和喹诺酮类等抗生素耐药基因。S1-PFGE和Southern印迹分析均为阴性。全基因组测序和比较分析表明，这两株分离株关系密切。
据我们所知，这项工作描述了首次在该细菌中检测到非质粒编码的NDM。本研究中分离出的该细菌具有广泛的耐药谱。表型和分子分析表明这两株分离株属于同一克隆。现在有必要对该菌种进行常规基因组监测，以有效遏制该地区耐碳青霉烯类细菌的进一步传播。
