属新种，种新，从. 科的海洋沉积物中分离得到。

gen. nov., sp. nov., isolated from marine sediment within the family .

机构信息

Marine College, Shandong University, Weihai, Shandong 264209, PR China.

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong 266237, PR China.

出版信息

Int J Syst Evol Microbiol. 2020 Nov;70(11):5654-5664. doi: 10.1099/ijsem.0.004458.

DOI:10.1099/ijsem.0.004458

PMID:32931410

Abstract

A Gram-stain-negative, aerobic coccus, designated CK1056, was isolated from coastal sediment of Xiaoshi Island, Weihai, PR China. Strain CK1056 was found to grow at 15-37 °C (optimum, 30 °C), with 0.5-6.5 % (w/v) NaCl (optimum, 3.5 %) and displayed alkaliphilic growth within the pH range of pH 6.5-10.0 (optimum, pH 8.0). The major fatty acids identified were iso-C and summed feature 3 (C 7 and/or C 6). The main polar lipids consisted of aminophosphoglycolipid and phosphatidylethanolamine. The predominant respiratory quinone was MK-7. The G+C content of the genomic DNA was 54.0 mol%. The result of the 16S rRNA gene sequence analysis confirmed the affiliation of this micro-organism to the family , with KCTC 12865 as its closest relative with only 88.0 % sequence similarity. From the taxonomic data obtained in this study, we propose that the new marine isolate be placed into a novel species within a novel genus in the family , phylum , for which the name gen. nov., sp. nov. is proposed. The type strain is CK1056 (=KCTC 72798=MCCC 1H00425).

摘要

一株革兰氏阴性、需氧球菌，被命名为 CK1056，从中国威海小石岛的沿海沉积物中分离得到。菌株 CK1056 被发现能够在 15-37°C（最适温度为 30°C）下生长，可耐受 0.5-6.5%（w/v）的 NaCl（最适浓度为 3.5%），在 pH 6.5-10.0（最适 pH 为 8.0）的范围内呈现嗜碱性生长。鉴定出的主要脂肪酸为异-C 和总和特征 3（C7 和/或 C6）。主要的极性脂包括氨基磷酰甘油和磷脂酰乙醇胺。主要的呼吸醌为 MK-7。基因组 DNA 的 G+C 含量为 54.0 mol%。16S rRNA 基因序列分析的结果证实了该微生物与家族的归属关系，与 KCTC 12865 的亲缘关系最近，仅有 88.0%的序列相似度。根据本研究获得的分类学数据，我们建议将新的海洋分离株归入家族中的一个新属的新种，提议使用属名 gen. nov.，种名 sp. nov.来命名。模式菌株为 CK1056（=KCTC 72798=MCCC 1H00425）。