State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, PR China.
State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, PR China.
Int J Syst Evol Microbiol. 2021 Feb;71(2). doi: 10.1099/ijsem.0.004621.
A non-motile, Gram-staining negative, catalase- and oxidase-positive, crescent-rod shaped bacterium, designated strain CUG 91308, was isolated from a sediment sample of Qinghai Lake, Qinghai Province, China. Colonies on OSM agar were round, smooth, flat and pinkish-orange in colour. Strain CUG 91308 could grow at 15-37 °C, pH 6-12 and in the presence of up to 7.0 % NaCl (w/v). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CUG 91308 belonged to the family and formed a clade with the genus in the phylogenetic tree, but separated from any species of the known genera within the family. The genomic DNA G+C content is about 42.1 %. The predominant fatty acids (>10 %) were iso-C (21.1 %), summed feature 3 (C 7c / C 6c / iso-C 2OH) (14.3 %), iso-C 3OH (12.3 %) and summed feature 9 (iso-C 9c / C 10-methyl) (10.6 %). The polar lipids of strain CUG 91308 were phosphatidylethanolamine (PE) and four unidentified polar lipids. Strain CUG 91308 contained MK-7 as the major respiratory quinone. On the basis of phenotypic, genotypic and phylogenetic data, strain CUG 91308 represents a novel species of a novel genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the proposed new isolate is CUG 91308 (=KCTC 62636=CGMCC 1.13593).
一株非运动、革兰氏染色阴性、过氧化氢酶和氧化酶阳性、新月形棒状的细菌，被命名为 CUG 91308 菌株，从中国青海省青海湖的沉积物样本中分离得到。OSM 琼脂上的菌落为圆形、光滑、扁平，呈粉红色橙色。CUG 91308 株可在 15-37°C、pH6-12 和高达 7.0%（w/v）NaCl 的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 CUG 91308 属于 科，在系统发育树中与 属形成一个分支，但与该科内任何已知属的种都分离出来。基因组 DNA G+C 含量约为 42.1%。主要脂肪酸（>10%）为 iso-C（21.1%）、特征 3 总和（C7c/C6c/iso-C2OH）（14.3%）、iso-C3OH（12.3%）和特征 9 总和（iso-C9c/C10-methyl）（10.6%）。CUG 91308 株的极性脂为磷脂酰乙醇胺（PE）和四种未鉴定的极性脂。菌株 CUG 91308 含有 MK-7 作为主要呼吸醌。基于表型、基因型和系统发育数据，CUG 91308 株代表了一个新科、一个新属的新型种，建议采用新属名 ，新种名 。建议的新分离株的模式株为 CUG 91308（=KCTC 62636=CGMCC 1.13593）。
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