First Central Clinical College of Tianjin Medical University, 24 Fukang Road, Nankai District, Tianjin, China; Department of Radiology, Tianjin First Central Hospital, 24 Fukang Road, Nankai District, Tianjin, China.
Department of Transplantation, Tianjin First Central Hospital, 24 Fukang Road, Nankai District, Tianjin, China.
Magn Reson Imaging. 2020 Dec;74:105-112. doi: 10.1016/j.mri.2020.09.010. Epub 2020 Sep 12.
To assess the value of multiparametric magnetic resonance imaging including intravoxel incoherent motion (IVIM), diffusion tensor imaging (DTI) and blood oxygen level dependent (BOLD) MRI in differentiating the severity of hepatic warm ischemia-reperfusion injury (WIRI) in a rabbit model.
Fifty rabbits were randomly divided into a sham-operation group and four test groups (n = 10 for each group) according to different hepatic warm ischemia times. IVIM, DTI and BOLD MRI were performed on a 3 T MR scanner with 11 b values (0 to 800 s/mm), 2 b values (0 and 500 s/mm) on 12 diffusion directions, multiple-echo gradient echo (GRE) sequences (TR/TE, 75/2.57-24.25 ms), respectively. IVIM, DTI and BOLD MRI parameters, hepatic biochemical and histopathological parameters were compared. Pearson and Spearman correlation methods were performed to assess the correlation between these MRI parameters and laboratory parameters. Furthermore, receiver operating characteristic (ROC) curves were compiled to determine diagnostic efficacies.
True diffusion (Dslow), pseudodiffusion (Dfast), perfusion fraction (PF), mean diffusivity (MD) significantly decreased, while R2* significantly increased with prolonged warm ischemia times, and significant differences were found in all of biochemical and histopathological parameters (all P < 0.05). Dslow, PF, and R2* correlated significantly with all of biochemical and histopathological parameters (all |r| = 0.381-0.746, all P < 0.05). ROC analysis showed that the area under the ROC curve (AUC) of IVIM across hepatic WIRI groups was the largest among IVIM, DTI and BOLD.
Multiparametric MRI may be helpful with characterization of early changes and determination of severity of hepatic WIRI in a rabbit model.
评估磁共振多参数成像（包括体素内不相干运动成像（IVIM）、弥散张量成像（DTI）和血氧水平依赖成像（BOLD））在兔肝热缺血再灌注损伤（WIRI）严重程度中的价值。
50 只兔子随机分为假手术组和四组实验组（每组 10 只），根据不同的肝热缺血时间。在 3T 磁共振扫描仪上进行 IVIM、DTI 和 BOLD MRI 检查，采用 11 个 b 值（0 至 800s/mm）、2 个 b 值（0 和 500s/mm）、12 个弥散方向、多回波梯度回波（GRE）序列（TR/TE，75/2.57-24.25ms）。比较 IVIM、DTI 和 BOLD MRI 参数、肝生化和组织病理学参数。采用 Pearson 和 Spearman 相关方法评估这些 MRI 参数与实验室参数之间的相关性。此外，还绘制了受试者工作特征（ROC）曲线以确定诊断效能。
随着热缺血时间的延长，真扩散（Dslow）、假性扩散（Dfast）、灌注分数（PF）和平均扩散系数（MD）明显降低，而 R2明显增加，所有生化和组织病理学参数均有显著差异（均 P<0.05）。Dslow、PF 和 R2与所有生化和组织病理学参数均呈显著相关（|r|=0.381-0.746，均 P<0.05）。ROC 分析显示，IVIM 在肝 WIRI 组的 ROC 曲线下面积（AUC）最大。
磁共振多参数成像有助于兔肝热缺血再灌注损伤的早期变化特征和严重程度的评估。
