Department of Surgery, University Hospital of Ioannina and School of Medicine, University of Ioannina, Ioannina, Greece.
Experimental, Educational and Research Center ELPEN, Athens, Greece.
In Vivo. 2022 Jan-Feb;36(1):153-160. doi: 10.21873/invivo.12686.
Description of an anesthetic recovery model with endotracheal intubation in rabbits which provides metabolic stability for the study of the late phase of liver ischemia/reperfusion (I/R) injury.
Two groups of New Zealand rabbits, n=7 in each, were used: Ischemia/reperfusion (I/R) group (45 min of partial liver ischemia/reperfusion) and no intervention (sham) group. Blood alanine aminotransferase, lactate, pH values, mean arterial pressure and pCO were calculated at baseline, and at 2 and 24 h post reperfusion. Tissue samples from left (ischemic) and right (non-ischemic) liver lobes were examined at 2 and 24 h after reperfusion.
The I/R group presented significantly higher levels of alanine aminotransferase (p=0.001) at 2 and 24 h, and of lactate (p=0.016) at 2 h post reperfusion. No differences were documented for pH, mean arterial pressure and pCO Histological exanimation revealed significant injury at 24 h post reperfusion for the I/R group.
This anesthetic recovery model permitted avoidance of hypoxia and respiratory acidosis, allowing the study of the late phase of I/R injury.
描述一种兔气管内插管麻醉恢复模型，为肝缺血/再灌注（I/R）损伤晚期阶段的研究提供代谢稳定性。
使用两组新西兰兔，每组 n=7：缺血/再灌注（I/R）组（45 分钟部分肝缺血/再灌注）和无干预（假手术）组。在再灌注前、再灌注后 2 小时和 24 小时计算血丙氨酸转氨酶、乳酸、pH 值、平均动脉压和 pCO。在再灌注后 2 小时和 24 小时检查左（缺血）和右（非缺血）肝叶的组织样本。
I/R 组在再灌注后 2 小时和 24 小时时丙氨酸转氨酶（p=0.001）和再灌注后 2 小时时乳酸（p=0.016）水平显著升高。pH 值、平均动脉压和 pCO 无差异。组织学检查显示 I/R 组在再灌注后 24 小时有明显损伤。
这种麻醉恢复模型避免了缺氧和呼吸性酸中毒，允许研究 I/R 损伤的晚期阶段。
