描述一种用于研究肝脏缺血再灌注损伤晚期阶段的兔恢复模型。
Description of a Recovery Model in Rabbits for the Study of the Late Phase of Liver Ischemia-Reperfusion Injury.
机构信息
Department of Surgery, University Hospital of Ioannina and School of Medicine, University of Ioannina, Ioannina, Greece.
Experimental, Educational and Research Center ELPEN, Athens, Greece.
出版信息
In Vivo. 2022 Jan-Feb;36(1):153-160. doi: 10.21873/invivo.12686.
AIM
Description of an anesthetic recovery model with endotracheal intubation in rabbits which provides metabolic stability for the study of the late phase of liver ischemia/reperfusion (I/R) injury.
MATERIALS AND METHODS
Two groups of New Zealand rabbits, n=7 in each, were used: Ischemia/reperfusion (I/R) group (45 min of partial liver ischemia/reperfusion) and no intervention (sham) group. Blood alanine aminotransferase, lactate, pH values, mean arterial pressure and pCO were calculated at baseline, and at 2 and 24 h post reperfusion. Tissue samples from left (ischemic) and right (non-ischemic) liver lobes were examined at 2 and 24 h after reperfusion.
RESULTS
The I/R group presented significantly higher levels of alanine aminotransferase (p=0.001) at 2 and 24 h, and of lactate (p=0.016) at 2 h post reperfusion. No differences were documented for pH, mean arterial pressure and pCO Histological exanimation revealed significant injury at 24 h post reperfusion for the I/R group.
CONCLUSION
This anesthetic recovery model permitted avoidance of hypoxia and respiratory acidosis, allowing the study of the late phase of I/R injury.
目的
描述一种兔气管内插管麻醉恢复模型，为肝缺血/再灌注（I/R）损伤晚期阶段的研究提供代谢稳定性。
材料和方法
使用两组新西兰兔，每组 n=7：缺血/再灌注（I/R）组（45 分钟部分肝缺血/再灌注）和无干预（假手术）组。在再灌注前、再灌注后 2 小时和 24 小时计算血丙氨酸转氨酶、乳酸、pH 值、平均动脉压和 pCO。在再灌注后 2 小时和 24 小时检查左（缺血）和右（非缺血）肝叶的组织样本。
结果
I/R 组在再灌注后 2 小时和 24 小时时丙氨酸转氨酶（p=0.001）和再灌注后 2 小时时乳酸（p=0.016）水平显著升高。pH 值、平均动脉压和 pCO 无差异。组织学检查显示 I/R 组在再灌注后 24 小时有明显损伤。
结论
这种麻醉恢复模型避免了缺氧和呼吸性酸中毒，允许研究 I/R 损伤的晚期阶段。
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