Division of Environmental Pathology, Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental University, 82 Inaoka-cho, Yokosuka, Kanagawa, 238-8580, Japan.
Division of Environmental Pathology, Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental University, 82 Inaoka-cho, Yokosuka, Kanagawa, 238-8580, Japan.
J Oral Biosci. 2020 Dec;62(4):327-335. doi: 10.1016/j.job.2020.08.003. Epub 2020 Sep 12.
Salivary glands produce brain-derived neurotrophic factor (BDNF), which increases plasma BDNF content. Salivary BDNF influences the hippocampus and enhances anxiety-like behaviors. Dyslipidemia affects the brain, promoting depression and anxiety-like behaviors. This study was performed to investigate whether hypertriglyceridemia influences salivary BDNF expression.
Hypertriglyceridemia was induced in rats by high-fat diet intake for 10 weeks. BDNF protein levels in the saliva and submandibular glands were measured using enzyme-linked immunosorbent assay (ELISA). Bdnf mRNA levels in the submandibular gland were determined using real-time polymerase chain reaction.
A hypertriglyceridemia rat model was established. Body weight did not differ between the control and hypertriglyceridemia groups. Bdnf mRNA and protein expression was increased in the submandibular gland in the hypertriglyceridemia group compared to the control group. BDNF expression was also significantly increased in the saliva of the hypertriglyceridemia group.
This is first study to show that hypertriglyceridemia induces BDNF expression in the rat submandibular gland and suggests that salivary BDNF is associated with lipid metabolism.
唾液腺产生脑源性神经营养因子（BDNF），增加血浆 BDNF 含量。唾液 BDNF 影响海马体并增强焦虑样行为。血脂异常影响大脑，促进抑郁和焦虑样行为。本研究旨在探讨高甘油三酯血症是否影响唾液 BDNF 表达。
通过高脂肪饮食喂养 10 周诱导大鼠高甘油三酯血症。使用酶联免疫吸附测定（ELISA）测量唾液和颌下腺中的 BDNF 蛋白水平。使用实时聚合酶链反应测定颌下腺中的 Bdnf mRNA 水平。
建立了高甘油三酯血症大鼠模型。对照组和高甘油三酯血症组之间的体重无差异。与对照组相比，高甘油三酯血症组颌下腺中的 Bdnf mRNA 和蛋白表达增加。高甘油三酯血症组的唾液中的 BDNF 表达也显著增加。
这是第一项表明高甘油三酯血症诱导大鼠颌下腺 BDNF 表达的研究，并提示唾液 BDNF 与脂代谢有关。
