College Of Agronomy and Zootechnics, Federal University of Mato Grosso (UFMT), Cuiabá, MT, Brazil; Faculty of Veterinary Medicine and Animal Science, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS, Brazil.
College Of Agronomy and Zootechnics, Federal University of Mato Grosso (UFMT), Cuiabá, MT, Brazil.
Anim Reprod Sci. 2020 Oct;221:106594. doi: 10.1016/j.anireprosci.2020.106594. Epub 2020 Sep 4.
The aim of this study was to evaluate induced reproduction in tambaqui females using buserelin acetate as compared with the traditional treatment regimen with carp pituitary extract (CPE). Reproductive traits of females with a body weight (BW) of 8.47 ± 1.52 kg were evaluated in ten females treated with buserelin acetate at the dose of 0.5 mL/kg BW, in a single application, and in ten females treated with CPE at the dose of 5.5 mg/kg BW, in two applications (10 % and 90 %, with a 12-h interval between applications). Spawning rate did not differ between the females treated with buserelin acetate (40 %) and CPE (40 %). Weight, fertilization rate and hatching rate did not differ between the two treatment groups. Degree-hours (determined as the average temperature multiplied by time, in hours, for spawning after the treatment with the second dose of CPE and after the single treatment with buserelin acetate) for spawning and number of oocytes per gram of gametes collected were greater (P < 0.05) in the females treated with buserelin acetate than in the females treated with CPE. Production index, absolute fecundity and relative fecundity were greater (P < 0.05) in the females treated with CPE. The hormone buserelin acetate promotes reproduction in tambaqui females with there being a similar spawning rate and oocyte quality, however, lesser production indices and fecundity than when there is the conventional treatment regimen imposed with carp pituitary extract.
本研究旨在评估使用促黄体生成素释放激素（buserelin acetate）与传统的鲤鱼脑垂体提取物（CPE）处理方案相比，对臀点脂鲤雌鱼进行诱导繁殖的效果。在 10 尾体重为 8.47±1.52kg 的雌鱼中，评估了单一剂量 0.5mL/kg BW 的促黄体生成素释放激素（buserelin acetate）和两次剂量 5.5mg/kg BW（10%和 90%，两次剂量之间间隔 12 小时）的 CPE 处理方案对雌鱼生殖特征的影响。使用促黄体生成素释放激素（buserelin acetate）处理的雌鱼的产卵率（40%）与 CPE 处理的雌鱼（40%）没有差异。两组处理的雌鱼的体重、受精率和孵化率没有差异。在第二次 CPE 剂量处理和促黄体生成素释放激素（buserelin acetate）单次处理后产卵所需的度小时数（确定为第二剂量 CPE 处理后和促黄体生成素释放激素（buserelin acetate）单次处理后平均温度乘以时间的小时数）和每克收集的配子中的卵母细胞数在使用促黄体生成素释放激素（buserelin acetate）处理的雌鱼中更高（P<0.05）。使用 CPE 处理的雌鱼的产卵指数、绝对繁殖力和相对繁殖力更高（P<0.05）。促黄体生成素释放激素（buserelin acetate）促进了臀点脂鲤雌鱼的繁殖，尽管产卵率和卵母细胞质量相似，但产卵指数和繁殖力低于传统的鲤鱼脑垂体提取物处理方案。
