Jugl Adam, Pekař Miloslav
Faculty of Chemistry, Brno University of Technology, Purkyňova 464, 612 00 Brno, Czech Republic.
Polymers (Basel). 2020 Sep 12;12(9):2069. doi: 10.3390/polym12092069.
High-resolution ultrasound spectroscopy and isothermal titration calorimetry were used to characterize interactions between hyaluronan and arginine oligomers. The molecular weight of arginine oligomer plays an important role in interactions with hyaluronan. Interactions were observable for arginine oligomers with eight monomer units and longer chains. The effect of the ionic strength and molecular weight of hyaluronan on interactions was tested. In an environment with increased ionic strength, the length of the arginine oligomer was crucial. Generally, sufficiently high ionic strength suppresses interactions between hyaluronan and arginine oligomers, which demonstrated interactions in water. From the point of view of the molecular weight of hyaluronan, the transition between the rod conformation and the random coil conformation appeared to be important.
高分辨率超声光谱法和等温滴定量热法被用于表征透明质酸与精氨酸寡聚物之间的相互作用。精氨酸寡聚物的分子量在与透明质酸的相互作用中起着重要作用。对于具有八个单体单元及更长链的精氨酸寡聚物，相互作用是可观察到的。测试了离子强度和透明质酸分子量对相互作用的影响。在离子强度增加的环境中，精氨酸寡聚物的长度至关重要。一般来说，足够高的离子强度会抑制透明质酸与精氨酸寡聚物之间的相互作用，而它们在水中表现出相互作用。从透明质酸分子量的角度来看，棒状构象和无规卷曲构象之间的转变似乎很重要。
