: A Novel Oncogenic Target of Lung Adenocarcinoma Regulated by Both Strands of ( and ).

Lung adenocarcinoma (LUAD) is the most aggressive cancer and the prognosis of these patients is unfavorable. We revealed that the expression levels of both strands of ( and ) were significantly suppressed in several cancer tissues. Analyses of large The Cancer Genome Atlas (TCGA) datasets showed that reduced or expression is associated with worse prognoses in LUAD patients (disease-free survival (DFS): = 0.1264 and 0.0316; overall survival (OS): = 0.0176 and 0.0756, respectively). Ectopic expression of these miRNAs attenuated LUAD cell proliferation, suggesting their tumor-suppressive roles. Our in silico analysis revealed 23 putative target genes of pre- in LUAD cells. Among these targets, high expressions of 19 genes were associated with worse prognoses in LUAD patients (OS: < 0.05). Notably, was regulated by both and in LUAD cells, and its aberrant expression was significantly associated with poor prognosis in LUAD patients (OS: = 0.0175; DFS: = 0.0276). knockdown using siRNAs suggested that elevated expression contributes to cancer progression. Aberrant FAM64A expression was detected in LUAD tissues by immunostaining. Taken together, our miRNA-based analysis might be effective for identifying prognostic and therapeutic molecules in LUAD.