• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一种进化癌症表观遗传学方法揭示了不同哺乳动物鳞状细胞癌中超保守非编码元件的 DNA 超甲基化。

An Evolutionary Cancer Epigenetic Approach Revealed DNA Hypermethylation of Ultra-Conserved Non-Coding Elements in Squamous Cell Carcinoma of Different Mammalian Species.

机构信息

Functional MR Unit, Bellaria Hospital, Department of Biomedical and Neuromotor Sciences, University of Bologna, 40139 Bologna, Italy.

Department of Veterinary Medical Sciences, University of Bologna, 40064 Ozzano Emilia, Italy.

出版信息

Cells. 2020 Sep 13;9(9):2092. doi: 10.3390/cells9092092.

DOI:10.3390/cells9092092
PMID:32933205
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7565279/
Abstract

BACKGROUND

Ultra-conserved non-coding elements (UCNEs) are genomic sequences that exhibit > 95% sequence identity between humans, mammals, birds, reptiles, and fish. Recent findings reported their functional role in cancer. The aim of this study was to evaluate the DNA methylation modifications of UNCEs in squamous cell carcinoma (SCC) from different mammal species.

METHODS

Fifty SCCs from 26 humans, 17 cats, 3 dogs, 1 horse, 1 bovine, 1 badger, and 1 porcupine were investigated. Fourteen feline stomatitis and normal samples from 36 healthy human donors, 7 cats, 5 dogs, 5 horses, 2 bovines and 1 badger were collected as normal controls. Bisulfite next generation sequencing evaluated the DNA methylation level from seven UCNEs (uc.160, uc.283, uc.416, uc.339, uc.270, uc.299, and uc.328).

RESULTS

57/59 CpGs were significantly different according to the Kruskal-Wallis test ( < 0.05) comparing normal samples with SCC. A common DNA hypermethylation pattern was observed in SCCs from all the species evaluated in this study, with an increasing trend of hypermethylation starting from normal mucosa, through stomatitis to SCC.

CONCLUSIONS

Our findings indicate that UCNEs are hypermethylated in human SCC, and this behavior is also conserved among different species of mammals.

摘要

背景

超保守非编码元件（UCNEs）是在人类、哺乳动物、鸟类、爬行动物和鱼类之间具有>95%序列同一性的基因组序列。最近的研究结果报告了它们在癌症中的功能作用。本研究旨在评估不同哺乳动物鳞状细胞癌（SCC）中 UCNEs 的 DNA 甲基化修饰。

方法

共检测了 26 例人类、17 例猫、3 例狗、1 例马、1 例牛、1 例獾和 1 例豪猪的 50 例 SCC。收集了 36 例健康人类供体、7 只猫、5 只狗、5 匹马、2 头牛和 1 只獾的 14 例猫口腔炎和正常样本作为正常对照。亚硫酸氢盐下一代测序评估了来自七个 UCNEs（uc.160、uc.283、uc.416、uc.339、uc.270、uc.299 和 uc.328）的 DNA 甲基化水平。

结果

Kruskal-Wallis 检验比较正常样本与 SCC 时，57/59CpG 存在显著差异（<0.05）。在所评估的所有物种的 SCC 中均观察到常见的 DNA 高甲基化模式，从正常黏膜开始，通过口腔炎到 SCC，高甲基化趋势增加。

结论

我们的研究结果表明，UCNEs 在人类 SCC 中呈高甲基化状态，这种行为在不同的哺乳动物物种中也是保守的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a598/7565279/8946125292a7/cells-09-02092-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a598/7565279/74b3a72cd47d/cells-09-02092-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a598/7565279/f7df8c3cda17/cells-09-02092-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a598/7565279/523d8790c4f4/cells-09-02092-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a598/7565279/3d2cd5d2762a/cells-09-02092-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a598/7565279/5ca490c9a965/cells-09-02092-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a598/7565279/ccffc7fadcd7/cells-09-02092-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a598/7565279/8946125292a7/cells-09-02092-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a598/7565279/74b3a72cd47d/cells-09-02092-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a598/7565279/f7df8c3cda17/cells-09-02092-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a598/7565279/523d8790c4f4/cells-09-02092-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a598/7565279/3d2cd5d2762a/cells-09-02092-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a598/7565279/5ca490c9a965/cells-09-02092-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a598/7565279/ccffc7fadcd7/cells-09-02092-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a598/7565279/8946125292a7/cells-09-02092-g007.jpg

相似文献

1
An Evolutionary Cancer Epigenetic Approach Revealed DNA Hypermethylation of Ultra-Conserved Non-Coding Elements in Squamous Cell Carcinoma of Different Mammalian Species.一种进化癌症表观遗传学方法揭示了不同哺乳动物鳞状细胞癌中超保守非编码元件的 DNA 超甲基化。
Cells. 2020 Sep 13;9(9):2092. doi: 10.3390/cells9092092.
2
CpG location and methylation level are crucial factors for the early detection of oral squamous cell carcinoma in brushing samples using bisulfite sequencing of a 13-gene panel.在使用13个基因面板的亚硫酸氢盐测序法对刷牙样本进行口腔鳞状细胞癌早期检测时，CpG位点和甲基化水平是关键因素。
Clin Epigenetics. 2017 Aug 15;9:85. doi: 10.1186/s13148-017-0386-7. eCollection 2017.
3
DNA methylation analysis by bisulfite next-generation sequencing for early detection of oral squamous cell carcinoma and high-grade squamous intraepithelial lesion from oral brushing.通过亚硫酸氢盐新一代测序进行DNA甲基化分析，以早期检测口腔鳞状细胞癌和来自口腔刷牙样本的高级别鳞状上皮内病变。
J Craniomaxillofac Surg. 2015 Oct;43(8):1494-500. doi: 10.1016/j.jcms.2015.07.028. Epub 2015 Aug 4.
4
Genome-wide DNA methylation profile identified a unique set of differentially methylated immune genes in oral squamous cell carcinoma patients in India.全基因组DNA甲基化谱在印度口腔鳞状细胞癌患者中鉴定出一组独特的差异甲基化免疫基因。
Clin Epigenetics. 2017 Feb 3;9:13. doi: 10.1186/s13148-017-0314-x. eCollection 2017.
5
Evolutionary conservation of DNA methylation in CpG sites within ultraconserved noncoding elements.CpG 位点中 DNA 甲基化在超保守非编码元件中的进化保守性。
Epigenetics. 2018;13(1):49-60. doi: 10.1080/15592294.2017.1411447. Epub 2018 Feb 6.
6
Pre-Operative Evaluation of DNA Methylation Profile in Oral Squamous Cell Carcinoma Can Predict Tumor Aggressive Potential.口腔鳞状细胞癌中 DNA 甲基化谱的术前评估可预测肿瘤侵袭潜能。
Int J Mol Sci. 2020 Sep 14;21(18):6691. doi: 10.3390/ijms21186691.
7
Alu hypomethylation in smoke-exposed epithelia and oral squamous carcinoma.暴露于烟雾的上皮组织和口腔鳞状细胞癌中的Alu低甲基化
Asian Pac J Cancer Prev. 2013;14(9):5495-501. doi: 10.7314/apjcp.2013.14.9.5495.
8
Suppressor microRNA-145 Is Epigenetically Regulated by Promoter Hypermethylation in Esophageal Squamous Cell Carcinoma.抑制性微小RNA-145在食管鳞状细胞癌中通过启动子高甲基化受到表观遗传调控。
Anticancer Res. 2015 Sep;35(9):4617-24.
9
Aberrantly hypermethylated tumor suppressor genes were identified in oral squamous cell carcinoma (OSCC).口腔鳞状细胞癌（OSCC）中存在异常高甲基化的肿瘤抑制基因。
Clin Epigenetics. 2019 Aug 12;11(1):116. doi: 10.1186/s13148-019-0715-0.
10
Global analysis of DNA methylation changes during progression of oral cancer.口腔癌进展过程中 DNA 甲基化变化的全局分析。
Oral Oncol. 2013 Nov;49(11):1033-42. doi: 10.1016/j.oraloncology.2013.08.005. Epub 2013 Sep 13.

引用本文的文献

1
Triple combination of lomustine, temozolomide and irradiation reduces canine glioma cell survival in vitro.洛莫司汀、替莫唑胺和放疗三联治疗可降低犬神经胶质瘤细胞体外存活率。
Vet Med Sci. 2023 Jul;9(4):1573-1583. doi: 10.1002/vms3.1181. Epub 2023 Jun 26.
2
Temozolomide is additive with cytotoxic effect of irradiation in canine glioma cell lines.替莫唑胺与放疗在犬神经胶质瘤细胞系中具有协同细胞毒作用。
Vet Med Sci. 2021 Nov;7(6):2124-2134. doi: 10.1002/vms3.620. Epub 2021 Sep 3.

本文引用的文献

1
Methylation Profile of X-Chromosome-Related Genes in Male Breast Cancer.男性乳腺癌中X染色体相关基因的甲基化谱
Front Oncol. 2020 Jun 17;10:784. doi: 10.3389/fonc.2020.00784. eCollection 2020.
2
Application of a non-invasive oral brushing procedure based on bisulfite sequencing of a 13-gene panel to study high-risk OSCC patients.应用基于 13 基因组合成亚硫酸氢盐测序的非侵入性口腔刷检方法，研究高危口腔鳞状细胞癌患者。
Cancer Biomark. 2020;28(4):499-510. doi: 10.3233/CBM-190422.
3
Analysis of DNA methylation and TP53 mutational status for differentiating feline oral squamous cell carcinoma from non-neoplastic mucosa: A preliminary study.
分析 DNA 甲基化和 TP53 突变状态以区分猫口腔鳞状细胞癌与非肿瘤性黏膜：初步研究。
Vet Comp Oncol. 2020 Dec;18(4):825-837. doi: 10.1111/vco.12624. Epub 2020 Jul 18.
4
13-gene DNA Methylation Analysis from Oral Brushing: A Promising Non Invasive Tool in the Follow-up of Oral Cancer Patients.口腔拭子的13基因DNA甲基化分析：口腔癌患者随访中一种有前景的非侵入性工具。
J Clin Med. 2019 Dec 2;8(12):2107. doi: 10.3390/jcm8122107.
5
Genetic Variations of Ultraconserved Elements in the Human Genome.人类基因组中超保守元件的遗传变异。
OMICS. 2019 Nov;23(11):549-559. doi: 10.1089/omi.2019.0156.
6
Prognostic impact of intra-field heterogeneity in oral squamous cell carcinoma.口腔鳞状细胞癌中瘤内异质性的预后影响。
Virchows Arch. 2020 Apr;476(4):585-595. doi: 10.1007/s00428-019-02656-z. Epub 2019 Aug 29.
7
EPIC-TABSAT: analysis tool for targeted bisulfite sequencing experiments and array-based methylation studies.EPIC-TABSAT：用于靶向亚硫酸氢盐测序实验和基于阵列的甲基化研究的分析工具。
Nucleic Acids Res. 2019 Jul 2;47(W1):W166-W170. doi: 10.1093/nar/gkz398.
8
Prevalence of p53 dysregulations in feline oral squamous cell carcinoma and non-neoplastic oral mucosa.猫口腔鳞状细胞癌和非肿瘤性口腔黏膜中 p53 失调的流行情况。
PLoS One. 2019 Apr 18;14(4):e0215621. doi: 10.1371/journal.pone.0215621. eCollection 2019.
9
PD-1 (PDCD1) promoter methylation in Merkel cell carcinoma: prognostic relevance and relationship with clinico-pathological parameters.Merkel 细胞癌中 PD-1（PDCD1）启动子甲基化：预后相关性及与临床病理参数的关系。
Mod Pathol. 2019 Sep;32(9):1359-1372. doi: 10.1038/s41379-019-0261-5. Epub 2019 Apr 11.
10
Association of Periodontitis with Oral Cancer: A Case-Control Study.牙周炎与口腔癌的关联：病例对照研究。
J Dent Res. 2019 May;98(5):526-533. doi: 10.1177/0022034519827565. Epub 2019 Feb 19.