Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, China.
Carbohydr Polym. 2020 Dec 1;249:116847. doi: 10.1016/j.carbpol.2020.116847. Epub 2020 Aug 6.
In this paper, several novel chitosan derivatives bearing benzenoid / heterocyclic moieties were synthesized via introducing aminobenzene and heterocyclic compounds onto carboxymethyl chitosan. The specific structures of chitosan derivatives were confirmed by FTIR, H NMR, and elemental analysis. Meanwhile, the antioxidant efficiencies of chitosan derivatives were assayed in vitro. In particular, all chitosan derivatives showed significant improvement in superoxide-radical scavenging activity and DPPH radical scavenging activity. Their antifungal activities against two plant pathogenic fungi (Colletotrichum lagenarium and Phomopsis asparagi) were estimated in vitro by hyphal measurement, and all products exhibited excellent antifungal activity. Besides, the cytotoxicity of them was also measured by CCK-8 in vitro on L929 cells, and all samples showed low cytotoxicity. The good biocompatibility and enhanced biological activity of new synthetic chitosan derivatives might be obvious advantages, while applied in wide range of applications as antifungal agents or antioxidants in food, medicine, cosmetics, and other fields.
在本文中，通过将芳香族/杂环化合物引入羧甲基壳聚糖，合成了几种新型的带有苯环/杂环结构的壳聚糖衍生物。壳聚糖衍生物的特定结构通过 FTIR、H NMR 和元素分析得到确认。同时，体外测定了壳聚糖衍生物的抗氧化效率。特别是，所有壳聚糖衍生物的超氧自由基清除活性和 DPPH 自由基清除活性均有显著提高。通过菌丝测量法体外评估了它们对两种植物病原真菌（炭疽菌和芦笋茎枯病菌）的抗真菌活性，所有产物均表现出优异的抗真菌活性。此外，通过 CCK-8 法在 L929 细胞上体外测定了它们的细胞毒性，所有样品的细胞毒性均较低。新型合成壳聚糖衍生物的良好生物相容性和增强的生物活性可能是明显的优势，可广泛应用于食品、医药、化妆品等领域的抗真菌剂或抗氧化剂。
