State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, University of Chinese Academy of Sciences, Chinese Academy of Science, Beijing 100085, China.
State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, University of Chinese Academy of Sciences, Chinese Academy of Science, Beijing 100085, China; Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, University of Chinese Academy of Sciences, Chinese Academy of Science, Beijing 100085, China.
J Environ Sci (China). 2020 Nov;97:11-18. doi: 10.1016/j.jes.2020.04.032. Epub 2020 May 31.
It is known that many kinds of fermentative antibiotics can be removed by temperature-enhanced hydrolysis from production wastewater based on their easy-to-hydrolyze characteristics. However, a few aminoglycosides are hard to hydrolyze below 100°C because of their stability expressed by high molecular energy gap (ΔE). Herein, removal of hard-to-hydrolyze kanamycin residue from production wastewater by hydrothermal treatment at subcritical temperatures was investigated. The results showed the reaction temperature had a significant impact on kanamycin degradation. The degradation half-life (t) was shortened by 87.17-fold when the hydrothermal treatment temperature was increased from 100°C to 180°C. The t of kanamycin in the N process was extended by 1.08-1.34-fold compared to that of the corresponding air process at reaction temperatures of 140-180°C, indicating that the reactions during hydrothermal treatment process mainly include oxidation and hydrolysis. However, the contribution of hydrolysis was calculated as 75%-98%, which showed hydrolysis played a major role during the process, providing possibilities for the removal of kanamycin from production wastewaters with high-concentration organic matrices. Five transformation products with lower antibacterial activity than kanamycin were identified using UPLC-QTOF-MS analysis. More importantly, hydrothermal treatment could remove 97.9% of antibacterial activity (kanamycin EQ, 1,109 mg/L) from actual production wastewater with COD around 100,000 mg/L. Furthermore, the methane production yield in anaerobic inhibition tests could be increased about 2.3 times by adopting the hydrothermal pretreatment. Therefore, it is concluded that hydrothermal treatment as a pretreatment technology is an efficient method for removing high-concentration hard-to-hydrolyze antibiotic residues from wastewater with high-concentration organic matrices.
已知，基于易水解的特性，很多发酵抗生素可以通过强化水解从生产废水中去除。然而，一些氨基糖苷类抗生素由于其高分子能量间隙（ΔE）所表现出的稳定性，在 100°C 以下难以水解。在此，研究了亚临界温度下水热处理去除生产废水中难水解的卡那霉素残留。结果表明，反应温度对卡那霉素降解有显著影响。当水热处理温度从 100°C 升高到 180°C 时，卡那霉素的降解半衰期（t）缩短了 87.17 倍。在 140-180°C 的反应温度下，N 过程中卡那霉素的 t 比相应的空气过程延长了 1.08-1.34 倍，表明水热处理过程中的反应主要包括氧化和水解。然而，水解的贡献被计算为 75%-98%，这表明水解在该过程中起主要作用，为去除高浓度有机基质生产废水中的卡那霉素提供了可能性。使用 UPLC-QTOF-MS 分析鉴定了 5 种具有比卡那霉素更低抗菌活性的转化产物。更重要的是，水热处理可以去除实际生产废水中 97.9%的抗菌活性（卡那霉素 EQ，1109mg/L），实际生产废水中 COD 约为 100000mg/L。此外，采用水热预处理可以使厌氧抑制试验中的甲烷产率提高约 2.3 倍。因此，可以得出结论，水热处理作为一种预处理技术，是去除高浓度难水解抗生素残留的有效方法，适用于高浓度有机基质的废水。
