Li Xi, Yao Yixin, Wang Xiaoxiao, An Chang, Gao Shanshan, Xiang Fangtao, Dong Yangli
Sichuan Institute for Food and Drug Control, Chengdu 611730, China.
Kangmei Pharmaceutical Co., Ltd., Shenzhen 518000, China.
J Anal Methods Chem. 2020 Aug 31;2020:8836184. doi: 10.1155/2020/8836184. eCollection 2020.
(Danggui, DG) is one of the most commonly prescribed traditional Chinese medicines. The organic components include phthalides and phenolic acids. Meanwhile, inorganic elements play an important role in clinical effect. DG and its different parts have different effects. There is no relevant report on the analysis of organic compounds and inorganic elements among them. Therefore, ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry was developed for the simultaneous determination of 13 organic components (8 phthalides and 5 phenolic acids), and 8 inorganic elements were determined by inductively coupled plasma mass spectrometry. The contents of 32 samples were analyzed by orthogonal partial least squares discrimination analysis, hierarchical cluster analysis, and least-significant difference of one-way analysis of variance. The results showed that the differences were significant among DG and its different parts. 11 difference markers (Ca, Z-ligustilide, Mg, Mn, Fe, Na, K, Cu, Zn, coniferyl ferulate, and senkyunolide A) were obtained by variable importance for the project. These difference markers were some different among DG and its different parts, especially Z-ligustilide, coniferyl ferulate, Mg, Zn, the differences were significant. This study can provide a reference for DG research.
当归（DG）是最常用的中药之一。其有机成分包括苯酞类和酚酸类。同时，无机元素在临床疗效中发挥着重要作用。当归及其不同部位具有不同的功效。目前尚无关于其中有机化合物和无机元素分析的相关报道。因此，建立了超高效液相色谱-三重四极杆质谱联用方法同时测定13种有机成分（8种苯酞类和5种酚酸类），并采用电感耦合等离子体质谱法测定8种无机元素。通过正交偏最小二乘法判别分析、层次聚类分析和单因素方差分析的最小显著差异对32个样品的含量进行分析。结果表明，当归及其不同部位之间差异显著。通过变量重要性投影获得了11个差异标志物（钙、Z-藁本内酯、镁、锰、铁、钠、钾、铜、锌、松柏基阿魏酸和蛇床子素A）。这些差异标志物在当归及其不同部位之间存在一些差异，尤其是Z-藁本内酯、松柏基阿魏酸、镁、锌，差异显著。本研究可为当归的研究提供参考。
