Institute of Pomology and Olericulture, College of Horticulture, Sichuan Agricultural University, Chengdu 611130, People's Republic of China.
J Agric Food Chem. 2020 Oct 14;68(41):11421-11433. doi: 10.1021/acs.jafc.0c04438. Epub 2020 Sep 30.
The newly released Donghong kiwifruit is a promising commercial cultivar. The dynamic changes of major phenolic compounds (flavonols, flavanols, phenolic acids, and anthocyanins) during the representative stages of fruit development and ripening of the Donghong kiwifruit were determined by high-performance liquid chromatography. The corresponding time-course transcriptional changes were evaluated using the combined analysis of RNA-Seq and qRT-PCR. The most predominant phenolic compound in the Donghong kiwifruit was epicatechin. Cyanidin 3--[2--(β-xylosyl)--galactoside] and cyanidin 3---galactoside were two essential anthocyanins detected. Candidate genes and pathways involved in phenolic compounds biosynthesis were highlighted. The structural genes (2, 51, and 52) and the transcription factor (74-2) were strongly associated with anthocyanin biosynthesis. 4-1 may be a novel transcription factor that reduces anthocyanin accumulation. Results from the study may be a very useful supplement to current knowledge of molecular mechanisms to elucidate coloration in the red-fleshed kiwifruit and could help breeders modify the kiwifruit germplasm.
新推出的东红猕猴桃是一种很有前途的商业品种。采用高效液相色谱法测定了东红猕猴桃果实发育和成熟过程中主要酚类化合物（类黄酮、黄烷醇、酚酸和花青素）的动态变化。利用 RNA-Seq 和 qRT-PCR 的联合分析评估了相应的时程转录变化。在东红猕猴桃中含量最丰富的酚类化合物是表儿茶素。检测到的两种主要的花青素是矢车菊素 3--[2--（β-木糖基）-半乳糖苷]和矢车菊素 3---半乳糖苷。突出了参与酚类化合物生物合成的候选基因和途径。结构基因（2、51 和 52）和转录因子（74-2）与花青素生物合成密切相关。4-1 可能是一种降低花青素积累的新型转录因子。该研究结果可能是对阐明红肉猕猴桃着色分子机制的现有知识的一个非常有用的补充，并有助于培育者改良猕猴桃种质资源。
