Esteban N V, Yergey A L, Liberato D J, Loughlin T, Loriaux D L
Laboratory of Theoretical and Physical Biology, National Institute of Child Health and Human Development, Bethesda, Maryland 20892.
Biomed Environ Mass Spectrom. 1988 Jun 1;15(11):603-8. doi: 10.1002/bms.1200151105.
A thermospray high-performance liquid chromatography/mass spectrometry method for the separation and quantification of cortisol in human serum has been developed. The technique does not require derivatization, allows for both qualitative and quantitative determinations, provides increased specificity not available from conventional high-performance liquid chromatography, and has a detection limit of 5 pmol on-column. This isotope dilution mass spectrometry method, using d3-cortisol as an internal standard, allows precise determinations even at low isotopic ratios (2%-5 mole%). Evidence that this technique can be applied to the quantification of serum cortisol and to the determination of daily cortisol production in humans is presented.
已开发出一种热喷雾高效液相色谱/质谱法，用于分离和定量测定人血清中的皮质醇。该技术无需衍生化，可进行定性和定量测定，比传统高效液相色谱具有更高的特异性，柱上检测限为5皮摩尔。这种以d3-皮质醇为内标物的同位素稀释质谱法，即使在低同位素比率（2%-5摩尔%）下也能进行精确测定。文中还给出了该技术可应用于血清皮质醇定量分析及人体每日皮质醇生成量测定的证据。
