Lee Hyunjun, Lee Ahyun, Seo Nari, Oh Jiwon, Kweon Oh-Kyeong, An Hyun Joo, Kim Jaehan
Department of Food and Nutrition, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea.
Graduate School of Analytical Science and Technology, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea.
Life (Basel). 2020 Sep 14;10(9):199. doi: 10.3390/life10090199.
Protein glycosylation is a post-translational modification that impacts on protein activity, stability, and interactions. It was sensitively altered by the cellular state and, therefore, is now used for a diagnostic or prognostic indicator of various human diseases such as cancer. To evaluate the clinical feasibility in the veterinary area, the N-glycan biomarkers were discovered from canine serum for the diagnosis of osteoarthritis (OA), which is one of the most common diseases of dogs. N-glycome was obtained from 20 μL of canine serum by the enzymatic cleavage followed by the purification and enrichment using solid-phase extraction. Independent compositions of 163 and 463 N-glycans were found from healthy control (n = 41) and osteoarthritis patients (n = 92), respectively. Initially, 31 of the potential biomarkers were screened by the -values below 1.0 × 10 from ANOVA. Then, the area under the curve (AUC) and the intensity ratio between OA patient and healthy control (P/C ratio) were calculated. Considering the diagnostic efficacy, the AUC bigger than 0.9 and the P/C ratio larger than 3.0 were used to discover 16 N-glycans as diagnostic biomarkers. Particularly, five of the diagnostic biomarkers were AUC above 0.99 and three of N-glycans had AUC 1.0. The results suggest a clear possibility for N-glycan biomarkers to be used as a clinical tool in the veterinary medical area enabling to provide objective and non-invasive diagnostic information.
蛋白质糖基化是一种翻译后修饰，会影响蛋白质的活性、稳定性和相互作用。它会随细胞状态而发生敏感变化，因此现在被用作多种人类疾病（如癌症）的诊断或预后指标。为了评估在兽医领域的临床可行性，从犬血清中发现了用于诊断骨关节炎（OA）的N-聚糖生物标志物，OA是犬类最常见的疾病之一。通过酶切从20μL犬血清中获得N-糖组，然后使用固相萃取进行纯化和富集。在健康对照（n = 41）和骨关节炎患者（n = 92）中分别发现了163种和463种独立组成的N-聚糖。最初，通过方差分析筛选出31种潜在生物标志物，其P值低于1.0×10。然后，计算曲线下面积（AUC）以及OA患者与健康对照之间的强度比（P/C比）。考虑到诊断效力，将AUC大于0.9且P/C比大于3.0作为标准，发现了16种N-聚糖作为诊断生物标志物。特别地，其中5种诊断生物标志物的AUC高于0.99，3种N-聚糖的AUC为1.0。结果表明，N-聚糖生物标志物有很大可能性作为兽医医学领域的临床工具，能够提供客观且非侵入性的诊断信息。
