Department of Health Science and Technology, GAIHST, Gachon University, Incheon 21999, Korea.
Department of Biomedical Engineering, Gachon University, 191 Hambakmoero, Yeonsu-gu, Incheon 21936, Korea.
Sensors (Basel). 2020 Sep 14;20(18):5237. doi: 10.3390/s20185237.
Label-free and real-time monitoring of the bacterial viability is essential for the accurate and sensitive characterization of the antibiotic effects. In the present study, we investigated the feasibility of the interdigitated and wave-shaped electrode (IWE) for monitoring the effect of tetracycline or kanamycin on () and methicillin-resistant (MRSA). The electrical impedance spectra of the IWE immersed in the culture media for bacterial growth were characterized in a frequency range of 10 Hz to 1 kHz. The capacitance index (CI) (capacitance change relevant with the bacterial viability) was used to monitor the antibiotic effects on the . and MRSA in comparison to the traditional methods (disk diffusion test and optical density (OD) measurement). The experimental results showed that the percentage of change in CI (PCI) for the antibiotic effect on MRSA was increased by 51.58% and 57.83% in kanamycin and control, respectively. In contrast, the PCI value decreased by 0.25% for tetracycline, decreased by 52.63% and 37.66% in the cases of tetracycline and kanamycin-treated , and increased 2.79% in the control, respectively. This study demonstrated the feasibility of the IWE-based capacitance sensor for the label-free and real-time monitoring of the antibiotic effects on and MRSA.
无标记实时监测细菌活力对于准确、灵敏地描述抗生素的作用至关重要。本研究探讨了叉指波状电极（IWE）用于监测四环素或卡那霉素对 （ ）和耐甲氧西林金黄色葡萄球菌（MRSA）影响的可行性。将 IWE 浸入培养基中以进行细菌生长，在 10 Hz 至 1 kHz 的频率范围内对其进行了阻抗谱特征分析。采用电容指数（CI）（与细菌活力相关的电容变化）来监测抗生素对 （ ）和 MRSA 的影响，并与传统方法（圆盘扩散试验和光密度（OD）测量）进行了比较。实验结果表明，与对照相比，卡那霉素和对照处理的 MRSA 中，CI 的变化百分比（PCI）分别增加了 51.58%和 57.83%。相比之下，四环素的 PCI 值降低了 0.25%，四环素和卡那霉素处理的 中分别降低了 52.63%和 37.66%，而对照则增加了 2.79%。本研究证明了基于 IWE 的电容传感器用于无标记实时监测抗生素对 （ ）和 MRSA 作用的可行性。
