Department of Physics, Yonsei University, Seoul 03722, Republic of Korea.
Department of Physics, Yonsei University, Seoul 03722, Republic of Korea; Nanomedical Graduate Program, Yonsei University, Seoul 03722, Republic of Korea.
Biosens Bioelectron. 2018 Apr 15;102:164-170. doi: 10.1016/j.bios.2017.11.010. Epub 2017 Nov 10.
To prevent spread of infection and antibiotic resistance, fast and accurate diagnosis of bacterial infection and subsequent administration of antimicrobial agents are important. However, conventional methods for bacterial detection and antibiotic susceptibility testing (AST) require more than two days, leading to delays that have contributed to an increase in antibiotic-resistant bacteria. Here, we report an aptamer-functionalized capacitance sensor array that can monitor bacterial growth and antibiotic susceptibility in real-time. While E. coli and S. aureus were cultured, the capacitance increased over time, and apparent bacterial growth curves were observed even when 10 CFU/mL bacteria was inoculated. Furthermore, because of the selectivity of aptamers, bacteria could be identified within 1h using the capacitance sensor array functionalized with aptamers. In addition to bacterial growth, antibiotic susceptibility could be monitored in real-time. When bacteria were treated with antibiotics above the minimum inhibitory concentration (MIC), the capacitance decreased because the bacterial growth was inhibited. These results demonstrate that the aptamer-functionalized capacitance sensor array might be applied for rapid ASTs.
为了防止感染和抗生素耐药性的传播，快速准确地诊断细菌感染并随后使用抗菌药物非常重要。然而，用于细菌检测和抗生素药敏试验（AST）的传统方法需要两天以上的时间，这导致了延迟，从而导致了抗生素耐药菌的增加。在这里，我们报告了一种适配子功能化电容传感器阵列，可实时监测细菌生长和抗生素敏感性。当培养大肠杆菌和金黄色葡萄球菌时，电容随时间增加，即使接种 10 CFU/mL 细菌，也可以观察到明显的细菌生长曲线。此外，由于适配体的选择性，使用与适配体功能化的电容传感器阵列可以在 1 小时内识别细菌。除了细菌生长外，还可以实时监测抗生素敏感性。当细菌受到高于最小抑菌浓度（MIC）的抗生素处理时，由于细菌生长受到抑制，电容会降低。这些结果表明，适配子功能化电容传感器阵列可用于快速 AST。
