Department of Physical Activity Research, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8636, Japan.
Department of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan.
Sci Rep. 2020 Sep 16;10(1):15221. doi: 10.1038/s41598-020-72245-7.
The relative contribution of diet to colorectal cancer (CRC) incidence is higher than that for other cancers. Animal models have revealed that Escherichia coli containing polyketide synthase (pks E. coli) in the gut participates in CRC development. The purpose of this cross-sectional study was to examine the relationship between dietary intake and the prevalence of pks E. coli isolated from the microbiota in faecal samples of 223 healthy Japanese individuals. Dietary intake was assessed using a previously validated brief-type self-administered diet history questionnaire. The prevalence of pks E. coli was evaluated using faecal samples collected from participants and specific primers that detected pks E. coli. The prevalence of pks E. coli was 26.9%. After adjusting for baseline confounders, the prevalence of pks E. coli was negatively associated with the intake of green tea (odds ratio [OR], 0.59 [95% confidence interval (CI) 0.30-0.88] per 100 g/1,000 kcal increment) and manganese (OR, 0.43 [95% CI 0.22-0.85] per 1 mg/1,000 kcal increment) and was positively associated with male sex (OR, 2.27 [95% CI 1.05-4.91]). While futher studies are needed to validate these findings, these results provide insight into potential dietary interventions for the prevention of CRC.
饮食对结直肠癌（CRC）发病率的影响比其他癌症更大。动物模型表明，肠道中含有聚酮合酶（pks E. coli）的大肠杆菌参与了结直肠癌的发展。本横断面研究旨在研究饮食摄入与从 223 名健康日本个体粪便样本中分离出的微生物群中 pks E. coli 患病率之间的关系。使用先前验证的简短型自我管理饮食历史问卷评估饮食摄入。使用从参与者收集的粪便样本和检测 pks E. coli 的特定引物评估 pks E. coli 的患病率。pks E. coli 的患病率为 26.9%。在校正基线混杂因素后，pks E. coli 的患病率与绿茶（OR，0.59 [95%置信区间（CI）0.30-0.88]，每 100 g/1,000 千卡增量）和锰（OR，0.43 [95% CI 0.22-0.85]，每 1 mg/1,000 千卡增量）的摄入量呈负相关，与男性（OR，2.27 [95% CI 1.05-4.91]）呈正相关。虽然需要进一步的研究来验证这些发现，但这些结果为预防 CRC 的潜在饮食干预提供了思路。
