Liao Qiliao, Guo Yajie, Zhou Jianshuang, Wan Yi, Carballar-Lejarazú Rebeca, Sheng Liangjing, Zhang Feiping, Wu Songqing, Zou Shuangquan
College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350000, China.
Key Laboratory of Integrated Pest Management in Ecological Forests, Fujian Province University, Fujian Agriculture and Forestry University, Fuzhou, 350000, China.
Curr Microbiol. 2020 Nov;77(11):3321-3329. doi: 10.1007/s00284-020-02196-9. Epub 2020 Sep 16.
This study measured the changes of microorganisms in the midgut and habitat niche of Rhynchophorus ferrugineus Olivier, an invasive quarantine pest, by Illumina sequencing. The bacterial diversity in the R. ferrugineus larvae midgut and their habitat niche was compared to the uninfected P. sylvestris. The Proteobacteria and Firmicutes occupied a dominant position in the R. ferrugineus midgut and infected P. sylvestris, while in the uninfected P. sylvestris the predominant bacterial phylum was the Cyanobacteria. Enterobacter, Dysgonomonas, and Entomoplasma were the dominant bacterial genera in R. ferrugineus midgut and also within the infected trees and uninfected trees with low relative abundance. These bacteria could be exploited as the biopesticide vector to control R. ferrugineus population. Besides, Sphingobacterium, Shinella, and Rhodobacter genera had the same distribution pattern in the infected and uninfected P. sylvestris, and these bacteria were not found in the midgut of R. ferrugineus. Interestingly, Paludibacter and Parabacteroides were only distributed in the wood fiber of the infected P. sylvestris, which could be used as potential microbial markers to detect if the palm plants are damaged by the R. ferrugineus. The results of this study will be beneficial to the development of control strategies for R. ferrugineus.
本研究通过Illumina测序测定了入侵检疫害虫红棕象甲（Rhynchophorus ferrugineus Olivier）中肠及栖息生态位中微生物的变化。将红棕象甲幼虫中肠及其栖息生态位中的细菌多样性与未感染的樟子松（Pinus sylvestris）进行了比较。变形菌门（Proteobacteria）和厚壁菌门（Firmicutes）在红棕象甲中肠和感染的樟子松中占据主导地位，而在未感染的樟子松中，主要的细菌门是蓝细菌门（Cyanobacteria）。肠杆菌属（Enterobacter）、 dysgonomonas菌属和昆虫支原体属（Entomoplasma）是红棕象甲中肠以及感染树木和未感染树木中相对丰度较低的优势细菌属。这些细菌可作为生物农药载体用于控制红棕象甲种群。此外，鞘氨醇杆菌属（Sphingobacterium）、新鞘氨醇菌属（Shinella）和红杆菌属（Rhodobacter）在感染和未感染的樟子松中具有相同的分布模式，且在红棕象甲中肠中未发现这些细菌。有趣的是，沼杆菌属（Paludibacter）和副拟杆菌属（Parabacteroides）仅分布在感染的樟子松的木纤维中，可作为潜在的微生物标记物来检测棕榈植物是否受到红棕象甲的侵害。本研究结果将有助于制定红棕象甲的防治策略。
