Wang Sibao, Dos-Santos André L A, Huang Wei, Liu Kun Connie, Oshaghi Mohammad Ali, Wei Ge, Agre Peter, Jacobs-Lorena Marcelo
CAS Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
Department of Molecular Microbiology and Immunology, Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA.
Science. 2017 Sep 29;357(6358):1399-1402. doi: 10.1126/science.aan5478. Epub 2017 Sep 28.
The huge burden of malaria in developing countries urgently demands the development of novel approaches to fight this deadly disease. Although engineered symbiotic bacteria have been shown to render mosquitoes resistant to the parasite, the challenge remains to effectively introduce such bacteria into mosquito populations. We describe a bacterium strain (AS1) isolated from ovaries that stably colonizes the mosquito midgut, female ovaries, and male accessory glands and spreads rapidly throughout mosquito populations. AS1 was genetically engineered for secretion of anti- effector proteins, and the recombinant strains inhibit development of in mosquitoes.
发展中国家疟疾的巨大负担迫切需要开发新的方法来对抗这种致命疾病。尽管工程共生细菌已被证明能使蚊子对疟原虫产生抗性，但如何有效地将此类细菌引入蚊子种群仍是一项挑战。我们描述了一种从卵巢分离出的菌株（AS1），它能稳定地定殖于蚊子的中肠、雌蚊卵巢和雄蚊附腺，并能在蚊子种群中迅速传播。AS1经过基因工程改造以分泌抗效应蛋白，重组菌株可抑制疟原虫在蚊子体内的发育。
