Inserm, UMRS935 ESTeam Paris Sud, Malignant and Therapeutic Stem Cell Models, Villejuif, France.
Service d'hématologie, APHP, GHU Paris Sud, Villejuif, France.
Methods Mol Biol. 2021;2179:341-351. doi: 10.1007/978-1-0716-0779-4_26.
Epithelial-Mesenchymal Transition (EMT) and its reciprocal Mesenchymal-Epithelial Transition (MET) occur naturally as a cycling process during embryonic and foetal development. The capacity of such iterative cycles to drive cell fate and cellular and molecular behaviour in physiology or pathology remains unclear. We describe here a protocol to induce successive cycles of EMT/MET in an untransformed human mammary epithelial cell line (MCF10A) as well as the necessary controls for cycle validation.
上皮-间充质转化（EMT）及其逆向的间充质-上皮转化（MET）在胚胎和胎儿发育过程中作为一个循环过程自然发生。这种迭代循环在生理或病理条件下驱动细胞命运和细胞及分子行为的能力尚不清楚。我们在此描述了一种诱导未转化的人乳腺上皮细胞系（MCF10A）中连续 EMT/MET 循环的方案，以及循环验证所需的对照。
