Departamento de Biologia Celular, Cinvestav-IPN, Av. IPN # 2508, San Pedro Zacatenco, Mexico, DF 07360, Mexico.
Int J Biochem Cell Biol. 2011 Dec;43(12):1782-91. doi: 10.1016/j.biocel.2011.08.017. Epub 2011 Sep 16.
Epidemiological studies and animal models suggest an association between high levels of dietary fat intake and an increased risk of developing breast cancer. Epithelial-mesenchymal-transition (EMT) is a process, by which epithelial cells are transdifferentiated to a mesenchymal state, and it has been implicated in cancer progression, including invasion and metastasis. Linoleic acid (LA) induces proliferation and invasion in breast cancer cells. However, the role of LA on the EMT process in human mammary epithelial cells remains to be studied. In the present study, we demonstrate that LA induces a transient down-regulation of E-cadherin expression, accompanied with an increase of Snail1, Snail2, Twist1, Twist2 and Sip1 expressions. Furthermore, LA induces FAK and NFκB activation, MMP-2 and -9 secretions, migration and invasion. In summary, our findings demonstrate, for the first time, that LA promotes an EMT-like process in MCF10A human mammary epithelial cells.
流行病学研究和动物模型表明，高脂肪饮食摄入与乳腺癌风险增加之间存在关联。上皮-间充质转化（EMT）是一个过程，通过这个过程，上皮细胞被转分化为间充质状态，它与癌症的进展有关，包括侵袭和转移。亚油酸（LA）可诱导乳腺癌细胞增殖和侵袭。然而，LA 对人乳腺上皮细胞 EMT 过程的作用仍有待研究。在本研究中，我们证明 LA 诱导 E-钙黏蛋白表达的短暂下调，同时伴随着 Snail1、Snail2、Twist1、Twist2 和 Sip1 表达的增加。此外，LA 诱导 FAK 和 NFκB 激活、MMP-2 和 MMP-9 分泌、迁移和侵袭。总之，我们的研究结果首次表明，LA 促进 MCF10A 人乳腺上皮细胞发生 EMT 样过程。
