Department of Developmental and Stem Cell Biology, Institut Pasteur, UMR3738, CNRS, Paris, France.
Cell Adhesion and Mechanics, Institut Jacques Monod, CNRS UMR7592, Paris Diderot University, Paris, France.
Methods Mol Biol. 2021;2179:415-425. doi: 10.1007/978-1-0716-0779-4_29.
Optogenetics uses light to manipulate protein localization or activity from subcellular to supra-cellular level with unprecedented spatiotemporal resolution. We used it to control the activity of the Cdc42 Rho GTPase, a major regulator of actin polymerization and cell polarity. In this chapter, we describe how to trigger and guide cell migration using optogenetics as a way to mimic EMT in an artificial yet highly controllable fashion.
光遗传学利用光在亚细胞到超细胞水平上操纵蛋白质的定位或活性，具有前所未有的时空分辨率。我们使用它来控制 Cdc42 Rho GTPase 的活性，Cdc42 Rho GTPase 是肌动蛋白聚合和细胞极性的主要调节因子。在本章中，我们描述了如何使用光遗传学触发和引导细胞迁移，从而以一种人为但高度可控的方式模拟 EMT。
