Department of Animal and Plant Sciences, The University of Sheffield, Sheffield, UK.
Department of Chemical and Biological Engineering, The University of Sheffield, Sheffield, UK.
Mol Ecol. 2020 Nov;29(22):4428-4441. doi: 10.1111/mec.15636. Epub 2020 Sep 30.
Despite holding a central role in fertilization, reproductive traits often show elevated rates of evolution and diversification. The rapid evolution of seminal fluid proteins (Sfps) within populations is predicted to cause mis-signalling between the male ejaculate and the female during and after mating resulting in postmating prezygotic (PMPZ) isolation between populations. Crosses between Drosophila montana populations show PMPZ isolation in the form of reduced fertilization success in both noncompetitive and competitive contexts. Here we test whether male ejaculate proteins produced in the accessory glands or ejaculatory bulb differ between populations using liquid chromatography tandem mass spectrometry. We find more than 150 differentially abundant proteins between populations that may contribute to PMPZ isolation, including a number of proteases, peptidases and several orthologues of Drosophila melanogaster Sfps known to mediate fertilization success. Males from the population that elicit the stronger PMPZ isolation after mating with foreign females typically produced greater quantities of Sfps. The accessory glands and ejaculatory bulb show enrichment for different gene ontology (GO) terms and the ejaculatory bulb contributes more differentially abundant proteins. Proteins with a predicted secretory signal evolve faster than nonsecretory proteins. Finally, we take advantage of quantitative proteomics data for three Drosophila species to determine shared and unique GO enrichments of Sfps between taxa and which potentially mediate PMPZ isolation. Our study provides the first high-throughput quantitative proteomic evidence showing divergence of reproductive proteins between populations that exhibit PMPZ isolation.
尽管在受精过程中起着核心作用，但生殖特征通常表现出较高的进化和多样化速率。预计种群内精液蛋白（Sfps）的快速进化会导致雄性精液与雌性在交配期间和之后的错误信号传递，从而导致种群间的交配后合子前（PMPZ）隔离。Drosophila montana 种群之间的杂交显示出 PMPZ 隔离的形式，表现在非竞争和竞争环境下的受精成功率降低。在这里，我们使用液相色谱串联质谱法测试了生殖附属腺或射精管中产生的雄性精液蛋白是否在种群之间存在差异。我们发现，种群之间存在 150 多种差异丰富的蛋白质，这些蛋白质可能有助于 PMPZ 隔离，包括许多蛋白酶、肽酶和几种已知介导受精成功率的 Drosophila melanogaster Sfps 的同源物。与外来雌性交配后引发更强 PMPZ 隔离的雄性通常产生更多的 Sfps。生殖附属腺和射精管显示出不同的基因本体论（GO）术语富集，射精管贡献了更多差异丰富的蛋白质。具有预测分泌信号的蛋白质比非分泌蛋白质进化得更快。最后，我们利用三种果蝇物种的定量蛋白质组学数据来确定 Sfps 在分类群之间的共享和独特的 GO 富集，以及哪些可能介导 PMPZ 隔离。我们的研究提供了第一个高通量定量蛋白质组学证据，表明表现出 PMPZ 隔离的种群之间生殖蛋白的分歧。
