Cilento G
Department of Biochemistry, Universidade de São Paulo, Brazil.
Experientia. 1988 Jul 15;44(7):572-6. doi: 10.1007/BF01953304.
Efficient excited state formation - much higher than that hitherto expected - may occur in organelles and in intact cells. Excited triplet states can be enzymatically generated in high yields by different routes. An example is the oxidation of isobutanal to acetone and formic acid, catalyzed by horseradish peroxidase. Other enzymatic systems that generate triplet carbonyls are linear aliphatic aldehydes when oxidized by peroxidase/O2, or the indole-3-acetic acid/peroxidase/O2-reaction. The latter is widespread in plants. This new field - photobiochemistry without light - has led to a growing awareness of the idea that cells may utilize excited states to trigger photochemical processes even in the dark. Such phenomena are of considerable importance, also for the understanding of weak photon emission from biological systems.
在细胞器和完整细胞中可能会发生高效的激发态形成，其效率比迄今预期的要高得多。激发三重态可以通过不同途径以高产率酶促生成。一个例子是辣根过氧化物酶催化异丁醛氧化为丙酮和甲酸。其他产生三重态羰基的酶促系统包括过氧化物酶/氧气氧化线性脂肪醛，或吲哚 - 3 - 乙酸/过氧化物酶/氧气反应。后者在植物中广泛存在。这个新领域——无光光生物化学——已使人们越来越意识到，细胞甚至在黑暗中也可能利用激发态来触发光化学过程。此类现象对于理解生物系统的微弱光子发射也具有相当重要的意义。
