Thomas T, Power B, Hudson P, Schreiber G, Dziadek M
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.
Dev Biol. 1988 Aug;128(2):415-27. doi: 10.1016/0012-1606(88)90303-x.
Specific cDNA and oligonucleotide probes were used to study the appearance of transthyretin mRNA in developing rat brain using Northern gel analysis, cytoplasmic dot hybridization, and in situ hybridization. Transthyretin mRNA in embryonic rat brain was found to be confined to the epithelial layer of the choroid plexus primordia appearing first in the fourth ventricle, followed by appearance in the lateral ventricles, and subsequently in the third ventricle. Transthyretin mRNA was localized in these cells from early stages of neuroepithelium differentiation, showing that it is a sensitive marker for the differentiation of the choroid plexus within the fetal brain.
使用特异性cDNA和寡核苷酸探针，通过Northern凝胶分析、细胞质斑点杂交和原位杂交技术，研究转甲状腺素蛋白mRNA在发育中的大鼠脑中的出现情况。发现胚胎大鼠脑中的转甲状腺素蛋白mRNA局限于脉络丛原基的上皮层，首先出现在第四脑室，随后出现在侧脑室，接着出现在第三脑室。转甲状腺素蛋白mRNA在神经上皮分化的早期阶段就定位于这些细胞中，表明它是胎儿脑内脉络丛分化的一个敏感标志物。
