Life Science Building, Department of Entomology, LSU Agricultural Center, Baton Rouge, LA.
B. Doran Building, Department of Biological and Agricultural Engineering, LSU Agricultural Center, Baton Rouge, LA.
J Econ Entomol. 2020 Dec 9;113(6):2739-2744. doi: 10.1093/jee/toaa199.
A meridic diet overlay bioassay using empty, positively charged zein nanoparticles ((+)ZNP) was performed on soybean looper (Chrysodeixis includens (Walker)), tobacco budworm (Heliothis virescens (F.)), and velvetbean caterpillar (Anticarsia gemmatalis Hübner) (Lepidoptera: Noctuidae). Assessment of effects on mortality and development weights 7 d after ingestion of (+)ZNP were evaluated on larvae of each species. Treatments involved different concentrations, with H. virescens and A. gemmatalis offered 0 and 3,800 ppm (+)ZNP, whereas C. includens colonies were offered 0, 630, 1,260, and 2,520 ppm (+)ZNP. Mortality of A. gemmatalis and C. includens increased after ingestion of the highest (+)ZNP concentrations, while H. virescens neonate mortality was unaffected. Neonate and third-instar weights of A. gemmatalis and C. includens, and neonate H. virescens, decreased with high (+)ZNP concentrations. Following mortality results from A. gemmatalis neonates, a concentration response test was performed using a range of (+)ZNP concentrations. The LC50 for A. gemmatalis was 1,478 ppm. The potential of (+)ZNP as a pest management tactic is discussed.
采用空的正电玉米醇溶蛋白纳米粒（(+)ZNP）对大豆夜蛾（Chrysodeixis includens (Walker)）、烟草夜蛾（Heliothis virescens (F.)）和绒泡桐尺蠖（Anticarsia gemmatalis Hübner）（鳞翅目：夜蛾科）进行了 meridic 饮食生物测定。评估了摄入 (+)ZNP 7 天后对每种幼虫的死亡率和发育体重的影响。处理涉及不同的浓度，H. virescens 和 A. gemmatalis 提供 0 和 3800 ppm（+）ZNP，而 C. includens 群体则提供 0、630、1260 和 2520 ppm（+）ZNP。摄入最高 (+)ZNP 浓度后，A. gemmatalis 和 C. includens 的幼虫死亡率增加，而 H. virescens 幼虫的死亡率则不受影响。A. gemmatalis 和 C. includens 的幼虫和三龄幼虫体重以及 H. virescens 的幼虫体重随着高 (+)ZNP 浓度的增加而下降。在 A. gemmatalis 幼虫死亡率的结果之后，进行了浓度反应测试，使用了一系列（+）ZNP 浓度。A. gemmatalis 的 LC50 为 1478 ppm。讨论了（+）ZNP 作为害虫管理策略的潜力。
