Marques L H, Castro B A, Rossetto J, Silva O A B N, Moscardini V F, Zobiole L H S, Santos A C, Valverde-Garcia P, Babcock J M, Rule D M, Fernandes O A
Dow AgroSciences Industrial Ltda, Av. Nações Unidas 14.171 - Diamond Tower, São Paulo, SP 04794-000, Brazil (
Dow AgroSciences LLC, 9330 Zionsville Rd., Indianapolis, IN 46268 (
J Econ Entomol. 2016 Aug;109(4):1922-8. doi: 10.1093/jee/tow153. Epub 2016 Jul 8.
Bacillus thuringiensis (Bt) event DAS-81419-2 (Conkesta technology) in soybean, Glycine max (L.) Merrill, expresses Cry1F and Cry1Ac proteins to provide protection from feeding by several lepidopteran pests. A total of 27 field experiments across nine locations were conducted from 2011 to 2015 in southern and central Brazil to characterize the efficacy of DAS-81419-2 soybean infested with Anticarsia gemmatalis Hübner (Lepidoptera: Erebidae), Chrysodeixis includens (Walker) (Lepidoptera: Noctuidae), Heliothis virescens (F.) (Lepidoptera: Noctuidae), and Spodoptera cosmioides (Walker) (Lepidoptera: Noctuidae) during vegetative (V4) and reproductive (R2 and R4) crop developmental stages. The efficacy of DAS-81419-2 was compared to that of a non-Bt isogenic variety managed with or without applications of commercial foliar insecticides for lepidopteran control. DAS-81419-2 soybean consistently experienced defoliation levels of 0.5% or less (compared with 20.05-56.74% in the non-Bt, nonsprayed treatment) and larval survival of < 0.1% in all four species across the vegetative and reproductive plant stages evaluated. The efficacy of DAS-81419-2 was significantly higher than commercial foliar insecticides applied to the non-Bt variety. DAS-81419-2 soybeans containing two highly effective Bt proteins are expected to be a more robust IRM tool compared to single-trait Bt technologies. The consistent efficacy of DAS-81419-2 soybeans across years, locations, and crop stages suggests that it will be a valuable product for management of hard-to-control key lepidopteran pests in South American soybean production.
大豆（Glycine max (L.) Merrill）中的苏云金芽孢杆菌（Bt）事件DAS - 81419 - 2（Conkesta技术）表达Cry1F和Cry1Ac蛋白，以抵御多种鳞翅目害虫的取食。2011年至2015年期间，在巴西南部和中部的九个地点共进行了27项田间试验，以评估DAS - 81419 - 2大豆在营养生长阶段（V4）和生殖生长阶段（R2和R4）遭受豆蚀叶野螟（Anticarsia gemmatalis Hübner，鳞翅目：灯蛾科）、银纹夜蛾（Chrysodeixis includens (Walker)，鳞翅目：夜蛾科）、烟芽夜蛾（Heliothis virescens (F.)，鳞翅目：夜蛾科）和眉纹夜蛾（Spodoptera cosmioides (Walker)，鳞翅目：夜蛾科）侵害时的防治效果。将DAS - 81419 - 2的防治效果与一种非Bt同基因品种进行比较，该品种在使用或不使用商业叶面杀虫剂防治鳞翅目害虫的情况下进行管理。在评估的营养生长和生殖生长阶段，DAS - 81419 - 2大豆的落叶率始终保持在0.5%或更低（相比之下，非Bt、未喷施处理的落叶率为20.05 - 56.74%），并且在所有四个物种中的幼虫存活率均低于0.1%。DAS - 81419 - 2的防治效果显著高于施用于非Bt品种的商业叶面杀虫剂。与单性状Bt技术相比，含有两种高效Bt蛋白的DAS - 81419 - 2大豆有望成为一种更强大的害虫综合防治工具。DAS - 81419 - 2大豆在多年、多地和不同作物阶段都具有一致的防治效果，这表明它将成为南美洲大豆生产中防治难以控制的主要鳞翅目害虫的有价值产品。
