Department of Earth, Environment and Life Science, University of Genoa, 16132 Genova, Italy.
Department of Experimemtal Medicine, University of Genoa, 16132 Genova, Italy.
Molecules. 2020 Sep 15;25(18):4237. doi: 10.3390/molecules25184237.
S-adenosylmethionine (SAMe) is an endogenous methyl donor derived from ATP and methionine that has pleiotropic functions. Most SAMe is synthetized and consumed in the liver, where it acts as the main methylating agent and in protection against the free radical toxicity. Previous studies have shown that the administration of SAMe as a supernutrient exerted many beneficial effects in various tissues, mainly in the liver. In the present study, we aimed to clarify the direct effects of SAMe on fatty acid-induced steatosis and oxidative stress in hepatic and endothelial cells. Hepatoma FaO cells and endothelial HECV cells exposed to a mixture of oleate/palmitate are reliable models for hepatic steatosis and endothelium dysfunction, respectively. Our findings indicate that SAMe was able to significantly ameliorate lipid accumulation and oxidative stress in hepatic cells, mainly through promoting mitochondrial fatty acid entry for β-oxidation and external triglyceride release. SAMe also reverted both lipid accumulation and oxidant production (i.e., ROS and NO) in endothelial cells. In conclusion, these outcomes suggest promising beneficial applications of SAMe as a nutraceutical for metabolic disorders occurring in fatty liver and endothelium dysfunction.
S-腺苷甲硫氨酸（SAMe）是一种内源性甲基供体，由三磷酸腺苷（ATP）和蛋氨酸衍生而来，具有多种功能。大多数 SAMe 在肝脏中合成和消耗，在肝脏中，它作为主要的甲基供体，并具有抵抗自由基毒性的作用。先前的研究表明，作为一种超级营养素，SAMe 在各种组织中发挥了许多有益的作用，主要是在肝脏中。在本研究中，我们旨在阐明 SAMe 对脂肪酸诱导的肝和内皮细胞脂肪变性和氧化应激的直接作用。暴露于油酸盐/棕榈酸盐混合物的肝癌 FaO 细胞和内皮 HECV 细胞分别是肝脂肪变性和内皮功能障碍的可靠模型。我们的研究结果表明，SAMe 能够显著改善肝细胞中的脂质积累和氧化应激，主要通过促进线粒体脂肪酸进入β-氧化和外部甘油三酯释放来实现。SAMe 还使内皮细胞中的脂质积累和氧化剂产生（即 ROS 和 NO）都得到了逆转。总之，这些结果表明，SAMe 作为一种营养保健品，对于脂肪肝和内皮功能障碍等代谢紊乱具有有希望的有益应用。
