Savelkoul H F, Lebman D A, Benner R, Coffman R L
Department of Cell Biology, Erasmus University, Rotterdam, The Netherlands.
J Immunol. 1988 Aug 1;141(3):749-55.
IL-4 is able to preferentially enhance murine IgE levels in the supernatant of LPS-stimulated T cell-depleted splenic B cell cultures. Clonal and quantitative analysis of this response revealed that this is due partly to a 14-fold increased IgE precursor frequency and partly to a three-fold increased clone size of IgE-secreting cells. IL-4 increased the precursor frequency and the clone size of IgM-secreting cells not more than twofold. Both the IgM and IgE response in LPS-stimulated B cells were completely inhibited by the addition of anti-IgM mAb (M41) to the cultures, indicating that the IgE-secreting clones developed as subclones from precursors that express IgM. These cells lacked expression of membrane-bound IgE up to day 5 of the culture. Application of feeder cells in these cultures resulted in an increased precursor frequency of IgE-secreting clones among LPS-reactive B cells that is due, partially, to IL-4 produced by the feeder cells.
白细胞介素-4能够优先提高脂多糖刺激的、去除T细胞的脾B细胞培养上清液中的小鼠IgE水平。对这种反应的克隆和定量分析表明，这部分归因于IgE前体细胞频率增加了14倍，部分归因于分泌IgE细胞的克隆大小增加了3倍。白细胞介素-4使分泌IgM细胞的前体细胞频率和克隆大小增加不超过2倍。向培养物中添加抗IgM单克隆抗体（M41）可完全抑制脂多糖刺激的B细胞中的IgM和IgE反应，这表明分泌IgE的克隆是从表达IgM的前体细胞发育而来的亚克隆。在培养的第5天之前，这些细胞缺乏膜结合IgE的表达。在这些培养物中应用饲养细胞会导致脂多糖反应性B细胞中分泌IgE克隆的前体细胞频率增加，这部分归因于饲养细胞产生的白细胞介素-4。
