Tsuji Y, Clausen H, Nudelman E, Kaizu T, Hakomori S, Isojima S
Biomembrane Institute, University of Washington, Seattle 98119.
J Exp Med. 1988 Jul 1;168(1):343-56. doi: 10.1084/jem.168.1.343.
The epitope structure of the human sperm antigen reacting with antibodies present in sera of infertile women has been studied using mAb H6-3C4, which produces immobilization of human sperm in the presence of complement. Another antibody, NUH2, which also induces human sperm immobilization, was used to substantiate the presence of a receptor on sperm involved in susceptibility to immobilization. Both antibodies defined type 2 chain polylactosamine structure. H6-3C4 is directed to internally located repetitive N-acetyllactosamine, i.e., sialyl-i, i, or fucosyl-i. NUH2 defines binary alpha 2----3 sialyl type 2 chain, i.e., sialyl-I. Thus, the presence of antibodies in the sera of infertile women directed to sperm lactosaminoglycan or lactosaminolipid could be the basis for infertility in these cases.
利用单克隆抗体H6-3C4研究了与不育女性血清中抗体发生反应的人精子抗原的表位结构，该抗体在补体存在的情况下可使人精子发生制动。另一种同样能诱导人精子制动的抗体NUH2，被用于证实精子上存在与制动易感性相关的受体。两种抗体均确定了2型链聚乳糖胺结构。H6-3C4针对内部定位的重复性N-乙酰乳糖胺，即唾液酸化-i、i或岩藻糖基化-i。NUH2确定了二元α2----3唾液酸化2型链，即唾液酸化-I。因此，不育女性血清中针对精子乳糖胺聚糖或乳糖胺脂的抗体的存在可能是这些病例中不育的原因。
