Noble Research Institute LLC, Ardmore, Oklahoma 73401.
Noble Research Institute LLC, Ardmore, Oklahoma 73401
Plant Physiol. 2020 Nov;184(3):1532-1548. doi: 10.1104/pp.20.00950. Epub 2020 Sep 17.
Iron-sulfur (Fe-S) clusters are inorganic cofactors that are present in all kingdoms of life as part of a large number of proteins involved in several cellular processes, including DNA replication and metabolism. In this work, we demonstrate an additional role for two Fe-S cluster genes in biotic stress responses in plants. Eleven Fe-S cluster genes, including the - () and its interactor (), when silenced in , compromised nonhost resistance to pv. T1. expression was induced by pathogens and salicylic acid. Arabidopsis () and mutants, with reduced or gene expression, respectively, showed increased susceptibility to both host and nonhost pathogen infection. Arabidopsis and overexpressor lines displayed decreased susceptibility to infection by host pathogen pv. DC3000. The overexpression line exhibited constitutive upregulation of several defense-related genes and enrichment of gene ontology terms related to immunity and salicylic acid responses. Our results demonstrate that NFS1 and its interactor FH are involved not only in nonhost resistance but also in basal resistance, suggesting a new role of the Fe-S cluster pathway in plant immunity.
铁硫（Fe-S）簇是无机辅因子，存在于所有生命领域，作为涉及多种细胞过程的大量蛋白质的一部分，包括 DNA 复制和代谢。在这项工作中，我们证明了两个 Fe-S 簇基因在植物生物胁迫反应中的额外作用。十一个 Fe-S 簇基因，包括（）及其相互作用蛋白（），在沉默时，会损害对非宿主的抗性。表达受病原体和水杨酸诱导。拟南芥（）和突变体，分别降低或基因表达，对宿主和非宿主病原体感染的敏感性增加。拟南芥和过表达系对宿主病原体的感染表现出较低的敏感性。过表达系表现出几种与防御相关基因的组成性上调，并富集与免疫和水杨酸反应相关的基因本体论术语。我们的结果表明，NFS1 及其相互作用蛋白 FH 不仅参与非宿主抗性，还参与基础抗性，这表明 Fe-S 簇途径在植物免疫中具有新的作用。
