Department of Chemistry and Biochemistry, UMass Cranberry Health Research Center, University of Massachusetts Dartmouth, 285 Old Westport Road, North Dartmouth, MA 02747, USA.
Chem Commun (Camb). 2020 Oct 25;56(83):12578-12581. doi: 10.1039/d0cc03643a. Epub 2020 Sep 18.
We report the facile synthesis and characterization of a novel fluorescent α-amino acid 4-phenanthracen-9-yl-l-phenylalanine (Phen-AA) (5) that emits greenish blue light in the visible region. This genetically encodable l-α-amino acid has excellent photostability with a 75% quantum yield. It readily gets into human cells, being clearly imaged upon 405 nm laser excitation. The synthetic procedure is resistant to racemization and only involves three simple steps which use mild conditions and generate the Phen-AA in reasonably good yield. It may find broad applications in research, biotechnology, and the pharmaceutical industry.
我们报告了一种新型荧光α-氨基酸 4-菲-9-基-L-苯丙氨酸（Phen-AA）（5）的简便合成与表征，该氨基酸在可见区域发出蓝绿光。这种可遗传编码的 L-α-氨基酸具有极好的光稳定性，量子产率为 75%。它很容易进入人体细胞，在 405nm 激光激发下可清晰成像。该合成方法不易消旋，仅涉及三个简单步骤，条件温和，可合理获得 Phen-AA。它可能在研究、生物技术和制药行业有广泛的应用。
