Institut GReD, Université Clermont Auvergne, CNRS, Inserm, Clermont-Ferrand, 63000, France.
Methods Mol Biol. 2021;2214:1-10. doi: 10.1007/978-1-0716-0958-3_1.
A couple of days after fertilization of a mouse oocyte by a sperm, two sequential cell differentiation events segregate pluripotent cells that can be identified by the presence of specific markers. Early mammalian embryos are relatively easy to recover as they are not yet implanted in the uterus matrix. Several decades of experimentation have enabled to find appropriate media to culture them, and therefore provide an excellent way to test different experimental setups such as the use of signaling inhibitors. We provide here a commonly used protocol to culture preimplantation embryos as well as a method to detect pluripotent cells in blastocysts.
在精子使老鼠卵受精后的几天，两个连续的细胞分化事件将多能细胞分离出来，这些细胞可以通过特定标记物的存在来识别。早期哺乳动物胚胎相对容易回收，因为它们尚未植入子宫基质中。几十年的实验使人们能够找到合适的培养基来培养它们，因此提供了一种极好的方法来测试不同的实验设置，例如使用信号抑制剂。我们在这里提供了一种常用的培养着床前胚胎的方案，以及一种在囊胚中检测多能细胞的方法。
