Department of Pediatric Cardiology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, China.
Department of Pediatric Cardiology, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, China.
FEBS Lett. 2020 Dec;594(24):4307-4319. doi: 10.1002/1873-3468.13930. Epub 2020 Oct 12.
Transcriptional regulation participates in heart development. However, the transcriptomes of human embryonic hearts during Carnegie stage (CS)10-CS16 have not been elucidated. Here, we found marked changes in the morphology and transcriptome of the human embryonic heart from CS10 to CS11. At CS12-CS14, the embryonic heart undergoes hypoxia-to-aerobic transformation. At CS14-CS16, transcriptome functions were related to energy metabolism, regulation of cholesterol, and processes related to inorganic substances. Moreover, the transcriptomes of cardiac progenitor cells derived from human embryonic stem cells (hESCs) most overlapped with those of human embryonic hearts at CS10. Cardiomyocytes derived from hESCs considerably overlapped with embryonic hearts at CS14-CS16. Overall, these results provide a new perspective into the characteristics of human embryonic heart development.
转录调控参与心脏发育。然而，卡内基阶段（CS）10-CS16 期间人类胚胎心脏的转录组尚未阐明。在这里，我们发现人类胚胎心脏从 CS10 到 CS11 的形态和转录组发生了明显变化。在 CS12-CS14 期间，胚胎心脏经历缺氧到有氧的转变。在 CS14-CS16 期间，转录组功能与能量代谢、胆固醇调节以及与无机物质相关的过程有关。此外，源自人类胚胎干细胞（hESC）的心肌细胞与 CS10 的人类胚胎心脏的转录组最为重叠。源自 hESC 的心肌细胞与 CS14-CS16 的胚胎心脏重叠程度相当高。总体而言，这些结果为人类胚胎心脏发育的特征提供了新的视角。
