Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510640, China.
Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510640, China.
Curr Biol. 2020 Nov 16;30(22):4432-4440.e4. doi: 10.1016/j.cub.2020.08.080. Epub 2020 Sep 17.
Finding a suitable oviposition site is a challenging task for a gravid female fly, because the hatched maggots have limited mobility, making it difficult to find an alternative host. The oriental fruit fly, Bactrocera dorsalis, oviposits on many types of fruits. Maggots hatching in a fruit that is already occupied by conspecific worms will face food competition. Here, we showed that maggot-occupied fruits deter B. dorsalis oviposition and that this deterrence is based on the increased β-caryophyllene concentration in fruits. Using a combination of bacterial identification, volatile content quantification, and behavioral analyses, we demonstrated that the egg-surface bacteria of B. dorsalis, including Providencia sp. and Klebsiella sp., are responsible for this increase in the β-caryophyllene contents of host fruits. Our research shows a type of tritrophic interaction between micro-organisms, insects, and insect hosts, which will provide considerable insight into the evolution of insect behavioral responses to volatile compounds.
寻找合适的产卵场所对怀孕的雌蝇来说是一项艰巨的任务，因为刚孵化的蛆移动能力有限，很难找到替代的宿主。东方果实蝇，Bactrocera dorsalis，在许多种类的水果上产卵。在已经被同种幼虫占据的水果中孵化的幼虫将面临食物竞争。在这里，我们表明，被幼虫占据的果实会阻止 B. dorsalis 产卵，这种阻止作用是基于果实中β-石竹烯浓度的增加。通过细菌鉴定、挥发物含量定量和行为分析的结合，我们证明了 B. dorsalis 的卵面细菌，包括 Providencia sp. 和 Klebsiella sp.，是导致宿主果实中β-石竹烯含量增加的原因。我们的研究展示了微生物、昆虫和昆虫宿主之间的一种三营养相互作用，这将为昆虫对挥发性化合物的行为反应的进化提供重要的见解。
