皮肤中的糖基化胆固醇：细胞外葡萄糖脑苷脂酶的合成作用。
Glucosylated cholesterol in skin: Synthetic role of extracellular glucocerebrosidase.
作者信息
Boer Daphne E C, Mirzaian Mina, Ferraz Maria J, Nadaban Andreea, Schreuder Anne, Hovnanian Alain, van Smeden Jeroen, Bouwstra Joke A, Aerts Johannes M F G
机构信息
Medical Biochemistry Leiden Institute of Chemistry, Leiden University, Leiden, the Netherlands.
Leiden Academic Centre for Drug Research, Leiden University, Leiden, the Netherlands.
出版信息
Clin Chim Acta. 2020 Nov;510:707-710. doi: 10.1016/j.cca.2020.09.017. Epub 2020 Sep 16.
The existence of glucosylated cholesterol (GlcChol) in tissue has recently been recognized. GlcChol is generated from glucosylceramide (GlcCer) and cholesterol through transglucosylation by two retaining β-glucosidases, GBA and GBA2. Given the abundance of GBA, GlcCer and cholesterol in the skin's stratum corneum (SC), we studied the occurrence of GlcChol. A significant amount of GlcChol was detected in SC (6 pmol/mg weight). The ratio GlcChol/GlcCer is higher in SC than epidermis, 0.083 and 0.011, respectively. Examination of GlcChol in patients with Netherton syndrome revealed comparable levels (11 pmol/mg). Concluding, GlcChol was identified as a novel component in SC and is likely locally metabolized by GBA. The physiological function of GlcChol in the SC warrants future investigation.
组织中糖基化胆固醇（GlcChol）的存在最近已得到确认。GlcChol是由葡萄糖神经酰胺（GlcCer）和胆固醇通过两种保留型β-葡萄糖苷酶GBA和GBA2的转糖基化作用生成的。鉴于皮肤角质层（SC）中GBA、GlcCer和胆固醇含量丰富，我们研究了GlcChol的存在情况。在SC中检测到大量的GlcChol（6皮摩尔/毫克重量）。SC中GlcChol/GlcCer的比值高于表皮，分别为0.083和0.011。对Netherton综合征患者的GlcChol检测显示其水平相当（11皮摩尔/毫克）。结论是，GlcChol被确定为SC中的一种新成分，可能在局部被GBA代谢。GlcChol在SC中的生理功能值得未来进一步研究。
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