Neuro-computing & Neuro-robotics Research Group, Complutense University of Madrid, Spain.
Innovation Research Group, Institute for Health Research San Carlos Clinical Hospital (IdISSC), Madrid, Spain.
J Neural Eng. 2020 Oct 31;17(5):055003. doi: 10.1088/1741-2552/abb9c0.
Age-related macular degeneration is an incurable chronic neurodegenerative disease, causing progressive loss of the central vision and even blindness. Up-to-date therapeutic approaches can only slow down he progression of the disease.
Feasibility study for a multilayered, silk fibroin-based, 3D biohybrid retina.
Fabrication of silk fibroin-based biofilms; culture of different types of cells: retinal pigment epithelium, retinal neurons, Müller and mesenchymal stem cells ; creation of a layered structure glued with silk fibroin hydrogel.
In vitro evidence for the feasibility of layered 3D biohybrid retinas; primary culture neurons grow and develop neurites on silk fibroin biofilms, either alone or in presence of other cells cultivated on the same biomaterial; cell organization and cellular phenotypes are maintained in vitro for the seven days of the experiment.
3D biohybrid retina can be built using silk silkworm fibroin films and hydrogels to be used in cell replacement therapy for AMD and similar retinal neurodegenerative diseases.
年龄相关性黄斑变性是一种无法治愈的慢性神经退行性疾病，导致中心视力进行性丧失，甚至失明。目前的治疗方法只能减缓疾病的进展。
研究一种基于丝素蛋白的多层 3D 生物混合视网膜的可行性。
丝素蛋白基生物膜的制备；不同类型细胞的培养：视网膜色素上皮细胞、视网膜神经元、Müller 胶质细胞和间充质干细胞；用丝素蛋白水凝胶将分层结构粘在一起。
体外证据表明分层 3D 生物混合视网膜具有可行性；原代培养神经元在丝素蛋白生物膜上生长并发育出神经突，无论是单独培养还是与同一生物材料上培养的其他细胞一起培养；在实验的七天内，细胞组织和细胞表型在体外得到维持。
使用丝蚕素纤维蛋白膜和水凝胶构建 3D 生物混合视网膜，可用于 AMD 和类似的视网膜神经退行性疾病的细胞替代治疗。
