Egorova Darya O, Gorbunova Tatyana I, Pervova Marina G, Kir'yanova Tatyana D, Demakov Vitalyi A, Saloutin Viсtor I, Chupakhin Oleg N
Institute of Ecology and Genetics of Microorganisms, Perm, 614081, Russia.
I.Ya. Postovskii Institute of Organic Synthesis, Yekaterinburg, 620990, Russia.
J Hazard Mater. 2020 Dec 5;400:123328. doi: 10.1016/j.jhazmat.2020.123328. Epub 2020 Jun 26.
For the first time, investigations are is carried out for the interactions of hydroxylated polychlorobiphenyls (HO-PCBs) mixtures, which were obtained from PCBs commercially available under the trade name Sovol, with the Rhodococcus (R.) strains. It is established that the HO-PCBs mixtures containing basic products within the range of 83.2-95.8% cause a toxic effect on the growth of R. wratislaviensis KT112-7, R. wratislaviensis CH628, R. ruber P25 strains. The inhibitory concentration (IC50) was varied within the range of 30-490 mg/l. For the first time, it is found that the bacterial strains can use HO-PCBs as a source of carbon with no co-substrate added. The strains are shown to degrade 95.5-100% of the HO-PCBs mixtures at a concentration of 0.1 g/l during 14 days. It is demonstrated that HO-PCBs degrading occurs following the classical bacterial pathway of transforming biphenyl/PCB. However, the HO-PCBs metabolites, which are substituted benzoic acids, are not the final products of the transformation and are subjected to further degrading by the strains. Therefore, the R. wratislaviensis KT112-7, R. wratislaviensis CH628, and R. ruber P25 strains are shown to degrade the HO-PCBs mixtures efficiently and are found to be stable to their toxic action.
