I.Ya. Postovskii Institute of Organic Synthesis, Ural Branch, Russian Academy of Sciences, Yekaterinburg 620990, Russia.
Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, Perm 614081, Russia.
J Hazard Mater. 2021 May 5;409:124471. doi: 10.1016/j.jhazmat.2020.124471. Epub 2020 Nov 4.
A possibility of using a complex approach is considered to explain features of biodestruction of polychlorinated biphenyls (PCBs), which are known to be persistent organic pollutants. The approach comprises the following main stages: (i) chemical modification of chloroarenes by hydroxylation and (ii) bacterial degradation of the hydroxylated derivatives. This approach is applicable to individual trichlorobiphenyls (PCB 29, PCB 30) and to a widespread mixture Trikhlorbifenil (analog of Aroclor 1242 and Delor 103). As bacterial strain destructors, the Rhodococcus-strains (КТ112-7, СН628, P25) were used. It was established that the main metabolites of microbial biodegradation of both polychlorobiphenyls and their hydroxy derivatives are polychloro- and hydroxy(polychloro)benzoic acids, which allows an assumption to be made about possible further biodegradation of these compounds down to the products of the base exchange reaction in a cell: water, carbon dioxide and chlorine compounds. The study discusses the effect that the structure of PCBs congeners causes on the conversion by hydroxylation, on the biodegradation rate of both PCBs and their hydroxy derivatives, and on the metabolite formation levels.
考虑采用一种复杂的方法来解释多氯联苯（PCBs）生物降解的特点，这些物质是已知的持久性有机污染物。该方法包括以下主要阶段：（i）氯芳烃的羟化化学修饰和（ii）羟化衍生物的细菌降解。这种方法适用于单个三氯联苯（PCB 29、PCB 30）和广泛存在的混合物三氯联苯（Aroclor 1242 和 Delor 103 的类似物）。作为细菌破坏菌株，使用了 Rhodococcus 菌株（КТ112-7、СН628、P25）。研究结果表明，多氯联苯及其羟基衍生物微生物生物降解的主要代谢产物是多氯和羟基（多氯）苯甲酸，这使得人们可以假设这些化合物可能进一步生物降解到底物交换反应的产物：水、二氧化碳和氯化合物。研究讨论了 PCBs 同系物的结构对羟化转化、对 PCBs 及其羟基衍生物的生物降解速率以及代谢产物形成水平的影响。
