Department of Microbiology, Paraná State University of Londrina, C.P. 6001, 86051990, Paraná, Brazil.
Technological Federal University of Paraná, Paraná, Brazil.
J Mycol Med. 2020 Dec;30(4):101043. doi: 10.1016/j.mycmed.2020.101043. Epub 2020 Sep 10.
Candida tropicalis is an emerging fungal pathogen associated with high mortality. We aimed to compare adherence capability of C. tropicalis to polystyrene and epithelial cell lines (HeLa and Vero), and determine whether adherent blastoconidia is cell-type specific. Blastoconidia adhesion to epithelial cells and polystyrene were determined by crystal violet assay. The percentage of epithelial cells with adhered blastoconidia and the number of adhered blastoconidia per cell line were determined by light microscopy. The correlation between adhesion surfaces was assessed by Pearson's correlation coefficient. The adhesiveness of C. tropicalis to polystyrene was greater than that observed for ephitelial cells. High correlation values (r 0.9999222, p 0.007941) were found for the adhesion capability between biotic and polystyrene surface for isolates 100.10 (obtained from blood) and 335.07 (obtained from tracheal secretion). The number of adherent blastoconidia per HeLa cell was greater in comparison to that observed for Vero cells (P<0.05). Further, high correlation (r 1, p 0.0001) was found for the adhesion ability between HeLa cells and Vero cells. The results suggest a correlation of C. tropicalis adhesion capability among different surfaces, and that the adhesion to epithelial cells is specific to the cell type.
热带假丝酵母菌是一种与高死亡率相关的新兴真菌病原体。我们旨在比较热带假丝酵母菌对聚苯乙烯和上皮细胞系（HeLa 和 Vero）的黏附能力，并确定黏附的芽生孢子是否具有细胞类型特异性。通过结晶紫测定法测定芽生孢子对上皮细胞和聚苯乙烯的黏附能力。通过相差显微镜确定黏附于上皮细胞的芽生孢子的百分比和每个细胞系的黏附芽生孢子的数量。通过皮尔逊相关系数评估黏附表面之间的相关性。C. tropicalis 对聚苯乙烯的黏附性大于对上皮细胞的黏附性。对于从血液中获得的分离株 100.10 和从气管分泌物中获得的分离株 335.07，在生物和聚苯乙烯表面之间的黏附能力之间发现了高相关值（r 0.9999222，p 0.007941）。与 Vero 细胞相比，HeLa 细胞中黏附的芽生孢子数量更多（P<0.05）。此外，还发现了 HeLa 细胞和 Vero 细胞之间黏附能力之间的高相关性（r 1，p 0.0001）。结果表明，C. tropicalis 在不同表面之间的黏附能力存在相关性，并且对上皮细胞的黏附具有细胞类型特异性。
