Laboratory of Ecological Information, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Laboratory of Comparative Agricultural Science, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Pest Manag Sci. 2021 Feb;77(2):851-859. doi: 10.1002/ps.6089. Epub 2020 Oct 9.
A new physical control method using ultraviolet-B (UV-B) lamps and light-reflecting sheets (UV method) significantly suppressed a spider mite population on greenhouse strawberries. Although UV-B radiation may adversely affect the survival of phytoseiid mites, previous research has suggested that Neoseiulus californicus can improve its survival on exposure to UV-B irradiation by consuming antioxidants contained in tea and peach pollen. In this study, we evaluated strawberry pollen as an alternative food source for N. californicus and examined whether antioxidants in the pollen mitigated UV-B damage to N. californicus.
The fecundity of N. californicus females reared on Tetranychus urticae decreased on shifting their diet to pollen. By contrast, females reared continuously on strawberry pollen produced as many eggs as females reared continuously on T. urticae. Survival and fecundity after UV-B irradiation were higher in females on the pollen diet. Oxygen radical absorbance capacity analysis revealed that the high antioxidant activity of strawberry pollen was due to four hydroxycinnamoyl spermidine derivatives.
Strawberry pollen was an adequate alternative food source for N. californicus. Feeding on strawberry pollen, which contains spermidine derivatives with high antioxidant activity, mitigated UV-B damage. This shows the potential of combining the UV-method with N. californicus for controlling T. urticae in strawberries.
一种使用紫外线-B（UV-B）灯和反光板的新型物理控制方法（UV 方法）显著抑制了温室草莓上的红蜘蛛种群。虽然 UV-B 辐射可能会对植绥螨的生存产生不利影响，但之前的研究表明，加州新小绥螨可以通过食用茶叶和桃花粉中含有的抗氧化剂来提高其在 UV-B 照射下的生存能力。在这项研究中，我们评估了草莓花粉作为加州新小绥螨的替代食物来源，并研究了花粉中的抗氧化剂是否减轻了 UV-B 对加州新小绥螨的伤害。
将加州新小绥螨的饮食从捕食二斑叶螨改为花粉后，其雌性个体的繁殖力下降。相比之下，连续以草莓花粉为食的雌性个体产生的卵数与连续以二斑叶螨为食的雌性个体相同。在接受 UV-B 辐射后，以花粉为食的雌性个体的存活率和繁殖力更高。氧自由基吸收能力分析表明，草莓花粉的高抗氧化活性归因于四种羟基肉桂酰亚精胺衍生物。
草莓花粉是加州新小绥螨的充足替代食物来源。以含有高抗氧化活性的亚精胺衍生物的草莓花粉为食，减轻了 UV-B 对其的伤害。这表明将 UV 方法与加州新小绥螨相结合来控制草莓上的二斑叶螨具有潜力。
