Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Scientific Observing and Experimental Station of Crop Pest in Guiyang, Institute of Entomology, Ministry of Agriculture, Guizhou University, Guiyang, 550025, China.
Guizhou Provincial Engineering Research Center of Medical Resourceful Healthcare Products, Guiyang Healthcare Vocational University, Guiyang, 550081, People's Republic of China.
Exp Appl Acarol. 2023 Dec;91(4):571-584. doi: 10.1007/s10493-023-00860-w. Epub 2023 Oct 31.
Mass rearing of the predatory mite Neoseiulus californicus (McGregor) (Acari: Phytoseiidae) using natural (prey) methods is costly and laborious, limiting its application in the biological control of pests. A high-production, low-cost method using a prey substitute would help to relieve this problem. Oulenziella bakeri Hughes (Acari: Winterschmidtiidae) could be an alternative prey source, but studies on the reproductive parameters of N. californicus under rearing conditions are lacking. This study evaluated the potential of O. bakeri as an alternative prey in N. californicus rearing by comparing developmental parameters among N. californicus reared on three diets based on an age-stage two-sex life table. We found that the preoviposition period and developmental time of N. californicus did not vary based on diet. The fecundity of N. californicus adults reared on O. bakeri was 29.8 eggs per female, which was lower than that of adults reared on Tetranychus urticae Koch (Acari: Tetranychidae) (42.9 eggs per female); there was no significant difference between O. bakeri and apple pollen (30.2 eggs per female). The oviposition rate of mites fed on O. bakeri was 69% of that fed on T. urticae. Neoseiulus californicus reared on O. bakeri and apple pollen showed the same intrinsic rate of increase (0.25 per day), which was 86% of the rate of those fed on T. urticae. Compared with predatory mites reared on natural prey, N. californicus reared on O. bakeri had a high survival rate and good oviposition and population growth parameters, suggesting that O. bakeri is suitable for the rearing of N. californicus.
大量饲养捕食性螨虫 Neoseiulus californicus（McGregor）（蜱螨目：植绥螨科）采用天然（猎物）方法既昂贵又费力，限制了其在害虫生物防治中的应用。使用替代猎物的高产量、低成本方法将有助于缓解这个问题。Oulenziella bakeri Hughes（蜱螨目：Winterschmidtiidae）可能是一种替代猎物来源，但关于 N. californicus 在饲养条件下的繁殖参数的研究还很缺乏。本研究通过比较基于年龄-阶段-两性生命表的三种饲养 N. californicus 的饮食的发育参数，评估了 O. bakeri 作为 N. californicus 饲养替代猎物的潜力。我们发现，N. californicus 的预产卵期和发育时间不因饮食而异。饲养在 O. bakeri 上的 N. californicus 成虫的产卵量为 29.8 只雌虫，低于饲养在 Tetranychus urticae Koch（蜱螨目：叶螨科）上的成虫（42.9 只雌虫）；饲养在 O. bakeri 和苹果花粉上的成虫产卵量（30.2 只雌虫）之间没有显著差异。以 O. bakeri 为食的螨虫的产卵率为以 T. urticae 为食的螨虫的 69%。饲养在 O. bakeri 和苹果花粉上的 N. californicus 的内禀增长率（每天 0.25）与饲养在 T. urticae 上的 N. californicus 相同，为 86%。与饲养在天然猎物上的捕食性螨虫相比，饲养在 O. bakeri 上的 N. californicus 具有较高的存活率和良好的产卵和种群增长参数，表明 O. bakeri 适合 N. californicus 的饲养。
