Student Research Committee, School of Medicine, Babol University of Medical Sciences, Babol, Iran.
Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Ganjafrooz Street, Babol, IR, Iran.
Indian J Gastroenterol. 2020 Aug;39(4):405-410. doi: 10.1007/s12664-020-01059-2. Epub 2020 Sep 19.
MicroRNAs (miRNAs) can post-transcriptionally regulate gene expression and are involved in the immune response. Excessive immune response to the gut microbiota plays a major role in the pathogenesis of Crohn's disease (CD). Regarding the role of miRNAs in immune response, this study aimed to investigate the contribution of miRNAs in the pathogenesis of CD. A total of 53 participants, including 23 CD patients and 30 healthy controls (HCs) were enrolled in this study. miRNAs, including miR-21, miR-29a, miR-29b, miR-31, miR-146a, miR-155, miR-181a, and miR-181c were evaluated via TaqMan MicroRNA Assays. Among the eight miRNAs, the amounts of miR-146a and miR-21 were significantly decreased in the CD patients relative to HC subjects. Moreover, we showed that there was a negative correlation between miR-146a and Harvey-Bradshaw index (HBI), as well as a positive correlation of miR-21 and miR-29b with HBI. Under-expression of miR-146a and miR-21, which are critical for the regulatory function of regulatory T cells (Tregs), is remarkably associated with CD.
微小 RNA（miRNA）可以在转录后调控基因表达，并参与免疫反应。过度的免疫反应对肠道微生物群在克罗恩病（CD）的发病机制中起着主要作用。关于 miRNA 在免疫反应中的作用，本研究旨在探讨 miRNA 在 CD 发病机制中的贡献。本研究共纳入 53 名参与者，包括 23 名 CD 患者和 30 名健康对照（HC）。通过 TaqMan miRNA 检测评估了包括 miR-21、miR-29a、miR-29b、miR-31、miR-146a、miR-155、miR-181a 和 miR-181c 在内的 8 种 miRNA。在这 8 种 miRNA 中，CD 患者的 miR-146a 和 miR-21 含量明显低于 HC 组。此外，我们还表明，miR-146a 与 Harvey-Bradshaw 指数（HBI）呈负相关，而 miR-21 和 miR-29b 与 HBI 呈正相关。miR-146a 和 miR-21 的低表达，对于调节性 T 细胞（Tregs）的调节功能至关重要，与 CD 有显著相关性。
