Heo Mi Young, Park Ra-Mi, Kim Bit-Na, Nguyen Ngoc-Han Thi, Jang Hye Weon, Kim Yang-Hoon, Min Jiho
Graduate School of Semiconductor and Chemical Engineering, Jeonbuk National University, 567 Baekje-daero, deokjin-gu, Jeonju-si, Jeollabuk-do, 54896, Republic of Korea.
Department of Bioprocess Engineering, Jeonbuk National University, 567 Baekje-daero, deokjin-gu, Jeonju-si, Jeollabuk-do, 54896, Republic of Korea.
Mol Biotechnol. 2020 Dec;62(11-12):557-562. doi: 10.1007/s12033-020-00272-w. Epub 2020 Sep 19.
The study of senescence preservative on cut flowers helps boost the commercial value of flowers. Senescence in cut flower is associated with an increase of ethylene production, and is significantly influenced by ethylene pathway. This study was conducted to investigate whether S-adenosyl-L-methionine (SAM) and aminocyclopropane-1-carboxylic acid (ACC) involved in the ethylene synthesis process are correlated with the lysosome. The alterations of lysosome which was treated with the ethylene precursors ACC and SAM in HeLa cell using the confocal laser scanning microscope were investigated. According to the experimental results, the activity of lysosomes increased concentration dependently by ACC treatment, however, no change was observed by SAM treatment. In addition, Liquid chromatography-mass spectrometry (LC/MS) analysis was performed to confirm the effect of lysosomal enzyme (LE) extracted from egg white on ACC reduction, but no change was observed. On the contrary, to confirm the effect of ACC on lysosomes, lysosomes were extracted from HeLa cells treated with 5 mM ACC and confirmed by FE-SEM. The results showed that the size of lysosomes treated with ACC is larger than that of the control, which was treated with distilled water. The lysosomes in the control group were distributed in various ranges from 0 to 800 nm, but those treated with 5 mM ACC were in the range of 400 nm to 800 nm or more. Therefore, lysosomes had no effect on ACC, the precursor of ethylene, the aging hormone of cut flowers, however, ACC had effect on lysosomes.
衰老保鲜剂对切花的研究有助于提高花卉的商业价值。切花衰老与乙烯产量增加有关，且受乙烯途径的显著影响。本研究旨在探讨乙烯合成过程中涉及的S-腺苷-L-甲硫氨酸（SAM）和1-氨基环丙烷-1-羧酸（ACC）是否与溶酶体相关。利用共聚焦激光扫描显微镜研究了在HeLa细胞中用乙烯前体ACC和SAM处理的溶酶体的变化。根据实验结果，ACC处理使溶酶体活性呈浓度依赖性增加，而SAM处理未观察到变化。此外，进行了液相色谱-质谱（LC/MS）分析，以确认从蛋清中提取的溶酶体酶（LE）对ACC还原的影响，但未观察到变化。相反，为了确认ACC对溶酶体的影响，从用5 mM ACC处理的HeLa细胞中提取溶酶体，并通过场发射扫描电子显微镜（FE-SEM）进行确认。结果表明，用ACC处理的溶酶体尺寸大于用蒸馏水作为对照处理的溶酶体。对照组溶酶体分布在0至800 nm的不同范围内，而用5 mM ACC处理的溶酶体分布在400 nm至800 nm或更大范围内。因此，溶酶体对乙烯的前体ACC（切花衰老激素）没有影响，然而，ACC对溶酶体有影响。
